| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ID4 |
| Uniprot No | P47928 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-161aa |
| 氨基酸序列 | MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC KAAEAAADEP ALCLQCDMND CYSRLRRLVP TIPPNKKVSK VEILQHVIDY ILDLQLALET HPALLRQPPP PAPPHHPAGT CPAAPPRTPL TALNTDPAGA VNKQGDSILC R |
| 分子量 | 16.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ID4蛋白的3条模拟参考文献示例，基于领域内典型研究方向推测：
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1. **文献名称**：**"ID4 silencing by promoter hypermethylation in breast cancer: Implications for epithelial-mesenchymal transition"**
**作者**：Smith J, et al.
**期刊**：*Oncogene* (2016)
**摘要**：研究揭示了ID4基因在乳腺癌中因启动子甲基化而沉默的现象，重组人ID4蛋白的体外表达可抑制肿瘤细胞侵袭，表明ID4通过调控EMT通路发挥抑癌作用。
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2. **文献名称**：**"High-yield expression and purification of recombinant human ID4 protein in E. coli: Functional characterization of DNA-binding inhibition"**
**作者**：Brown K, et al.
**期刊**：*Protein Expression and Purification* (2018)
**摘要**：报道了利用大肠杆菌表达系统高效生产重组人ID4蛋白的方法，并通过体外实验证明该蛋白可竞争性抑制转录因子的DNA结合活性。
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3. **文献名称**：**"ID4 regulates neural stem cell self-renewal and differentiation through modulation of the Notch signaling pathway"**
**作者**：Lee S, et al.
**期刊**：*Developmental Biology* (2020)
**摘要**：研究发现重组人ID4蛋白通过拮抗Notch信号通路，促进神经干细胞分化为神经元，为ID4在中枢神经系统发育中的功能提供了机制解释。
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*注：以上文献信息为示例性假设，实际研究中需根据具体论文内容引用。建议通过PubMed或Google Scholar以“recombinant human ID4 protein”、“ID4 expression”等关键词检索最新文献。*
Recombinant human ID4 (Inhibitor of DNA binding 4) protein is a functionally engineered version of the naturally occurring ID4 protein, a member of the helix-loop-helix (HLH) transcriptional regulator family. The ID4 gene, located on chromosome 6p22.3. encodes a dominant-negative regulator that inhibits the DNA-binding activity of basic HLH (bHLH) transcription factors by forming non-functional heterodimers. It plays critical roles in embryonic development, cell differentiation, and tissue-specific gene expression, particularly in the nervous system, mammary glands, and kidneys.
ID4 is notable for its dual roles in cancer biology, acting as both a tumor suppressor and promoter depending on context. It modulates key pathways like Wnt/β-catenin, TGF-β, and Notch, influencing cell cycle progression, apoptosis, and epithelial-mesenchymal transition (EMT). Dysregulation of ID4 is linked to malignancies (e.g., breast, ovarian, and gastric cancers) and neurological disorders.
Recombinant ID4 proteins are typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. These proteins are vital tools for studying ID4’s interaction networks, epigenetic regulation (e.g., promoter methylation in cancer), and therapeutic targeting. Research using recombinant ID4 has advanced insights into its role in stem cell maintenance, differentiation blockade, and tissue regeneration, underscoring its potential in regenerative medicine and oncology.
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