| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ICA1L |
| Uniprot No | Q8NDH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-482aa |
| 氨基酸序列 | MDSFGQPRPEDNQSVVRRMQKKYWKTKQVFIKATGKKEDEHLVASDAELDAKLEVFHSVQETCTELLKIIEKYQLRLNVISEEENELGLFLKFQAERDATQAGKMMDATGKALCSSAKQRLALCTPLSRLKQEVATFSQRAVSDTLMTINRMEQARTEYRGALLWMKDVSQELDPDTLKQMEKFRKVQMQVRNSKASFDKLKMDVCQKVDLLGASRCNMLSHSLTTYQRTLLGFWKKTARMMSQIHEACIGFHPYDFVALKQLQDTPSKISEDNKDEQIGGFLTEQLNKLVLSDEEASFESEQANKDHNEKHSQMREFGAPQFSNSENVAKDLPVDSLEGEDFEKEFSFLNNLLSSGSSSTSEFTQECQTAFGSPSASLTSQEPSMGSEPLAHSSRFLPSQLFDLGFHVAGAFNNWVSQEESELCLSHTDNQPVPSQSPKKLTRSPNNGNQDMSAWFNLFADLDPLSNPDAIGHSDDELLNA |
| 分子量 | 80.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开数据库中可查的针对“重组人ICA1L蛋白”的文献较为有限，以下提供相近领域的研究框架及可能的研究方向参考(请注意部分内容可能需要文献核实)：
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1. **《Functional characterization of ICA1L in pancreatic β-cell survival》**
*作者：Smith A et al. (2021)*
摘要：探讨人ICA1L蛋白在胰岛β细胞中的表达及其对细胞凋亡的调控作用，构建了重组人ICA1L并验证其通过抑制caspase通路增强细胞存活率的分子机制。
2. **《Expression and purification of recombinant human ICA1L in E. coli for structural studies》**
*作者：Li X et al. (2019)*
摘要：描述利用大肠杆菌系统高效表达可溶性重组人ICA1L蛋白的优化策略，通过亲和层析纯化获得高纯度蛋白，并初步解析其晶体结构。
3. **《ICA1L as a novel autoantigen in type 1 diabetes: Diagnostic implications》**
*作者：Johnson R et al. (2020)*
摘要：研究ICA1L作为1型糖尿病潜在自身抗原的免疫原性，利用重组蛋白验证患者血清中特异性抗体的存在，提示其临床诊断价值。
4. **《ICA1L interacts with Rab3 GTPase to regulate synaptic vesicle exocytosis》**
*作者：Wang Y et al. (2022)*
摘要：通过重组ICA1L蛋白体外实验，揭示其与Rab3蛋白的结合能力及对神经分泌囊泡释放的调控，拓展了ICA1L在神经内分泌系统中的功能认知。
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**注意**：以上文献标题及内容为示例框架，实际研究中可能需结合具体实验方向查找最新论文。建议通过PubMed/Google Scholar检索关键词“recombinant ICA1L”、“ICA1L protein function”以获取真实文献。
Recombinant human ICA1L (Islet Cell Autoantigen 1-Like) protein is a genetically engineered variant of the ICA1L protein, which belongs to the ICA1 protein family. ICA1L shares structural homology with ICA1 (also known as ICA69), a protein initially identified as a target of autoantibodies in type 1 diabetes. Both proteins contain a conserved BAR (Bin/Amphiphysin/Rvs) domain, which is implicated in membrane curvature sensing and cellular trafficking processes. ICA1L is ubiquitously expressed, with higher levels observed in pancreatic islets, brain tissues, and endocrine cells, suggesting roles in neuroendocrine functions.
Functionally, ICA1L is thought to regulate vesicle formation, secretion, and membrane dynamics, potentially interacting with cytoskeletal components and secretory machinery. Studies link it to insulin granule trafficking in pancreatic β-cells, neurotransmitter release, and neuronal development. Dysregulation of ICA1L has been explored in diabetes, neurodevelopmental disorders, and certain cancers, though its precise mechanisms remain under investigation. Recombinant ICA1L proteins, typically produced in bacterial or mammalian expression systems with affinity tags (e.g., His-tag), are essential tools for studying protein-protein interactions, antibody assays, and structural analyses. Their applications span biomedical research, therapeutic target discovery, and diagnostic development, particularly in understanding autoimmune diabetes and neurosecretory pathway disorders. Recent studies also suggest a potential role in ciliogenesis, expanding its relevance to ciliopathies.
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