| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IBRDC2 |
| Uniprot No | Q7Z419 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302aa |
| 氨基酸序列 | MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAEAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVMWNRTQVVGILVGLGIIALVTSPLLLASPCIICCVCKSCRGKKKKHDPSTT |
| 分子量 | 60 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IBRDC2蛋白的3篇参考文献的概括(注：文献为示例性内容，可能不对应真实论文)：
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1. **文献名称**：*"Expression and Functional Analysis of Recombinant Human IBRDC2 in Apoptosis Regulation"*
**作者**：Smith J. et al.
**摘要**：该研究利用大肠杆菌系统成功表达并纯化了重组人IBRDC2蛋白，并发现其通过泛素化修饰p53蛋白增强后者稳定性，从而促进肿瘤细胞凋亡，提示其在癌症治疗中的潜在作用。
2. **文献名称**：*"Structural Insights into the Ubiquitin Ligase Activity of IBRDC2"*
**作者**：Zhang L. et al.
**摘要**：通过晶体结构解析发现，IBRDC2的IBR结构域是其与泛素结合的关键区域。体外酶活实验证实重组IBRDC2具有E3泛素连接酶活性，为开发靶向泛素化通路的药物提供了依据。
3. **文献名称**：*"Recombinant IBRDC2 Interaction with Parkin in Mitochondrial Quality Control"*
**作者**：Brown K. et al.
**摘要**：研究表明重组人IBRDC2与Parkin蛋白协同促进受损线粒体的泛素化标记，调控线粒体自噬，可能在神经退行性疾病如帕金森病中发挥关键作用。
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**注意**：以上文献为示例，实际研究中IBRDC2可能涉及不同命名或功能描述，建议通过学术数据库(如PubMed)以“RBBP6”(可能与IBRDC2相关)或“IBR domain protein”等关键词检索最新文献。
**Background of Recombinant Human IBRDC2 Protein**
Recombinant human IBRDC2 (IBR-domain-containing protein 2), also known as BIRC7 or livin, is a multifunctional protein implicated in cellular processes such as apoptosis regulation, cell cycle control, and stress response. As a member of the inhibitor of apoptosis (IAP) family, IBRDC2 contains a baculovirus IAP repeat (BIR) domain critical for its anti-apoptotic activity and a C-terminal IBR (in-between RING) domain linked to ubiquitin ligase activity. It primarily exerts its function by binding to caspases (e.g., caspase-3. -7. -9) to inhibit programmed cell death, thereby promoting cell survival under stress conditions.
IBRDC2 is expressed in embryonic tissues and certain cancers, where its overexpression correlates with tumor progression, therapy resistance, and poor prognosis. Recombinant IBRDC2 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and therapeutic potential. Research focuses on developing IBRDC2-targeted strategies, such as small-molecule inhibitors or antisense oligonucleotides, to sensitize cancer cells to apoptosis. Its dual role in survival signaling and ubiquitination pathways also makes it a compelling target for understanding cellular homeostasis and disease mechanisms.
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