| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IBRDC1 |
| Uniprot No | Q8TC41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-100aa |
| 氨基酸序列 | KVQLGQVEIKCPITECFEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQFVWCFKCHSPWHE |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IBRDC1蛋白的3篇假设性参考文献(注：IBRDC1蛋白研究较少，以下为模拟示例，实际文献需通过学术数据库检索)：
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1. **文献名称**：*Functional characterization of recombinant human IBRDC1 as an E3 ubiquitin ligase in DNA damage response*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人IBRDC1蛋白，证实其通过IBR结构域介导与泛素结合酶的特异性互作，并调控p53蛋白的泛素化降解，参与DNA损伤修复过程。
2. **文献名称**：*Structural analysis of IBRDC1 reveals a novel zinc-binding domain critical for its protease interaction*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析重组IBRDC1蛋白的三维结构，发现其IBR结构域含独特的锌离子结合位点，实验表明该位点对与caspase-3的互作及凋亡调控至关重要。
3. **文献名称**：*Recombinant IBRDC1 protein production in mammalian cells and its role in NF-κB signaling*
**作者**：Zhang R, et al.
**摘要**：利用HEK293细胞系统表达重组人IBRDC1.揭示其通过泛素化修饰IKKγ蛋白负调控NF-κB通路，并抑制炎症因子释放的分子机制。
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**备注**：实际研究中，IBRDC1(含IBR结构域的蛋白1)可能涉及泛素化、凋亡或信号通路，但公开文献较少。建议通过UniProt数据库(ID：Q9NVP4)或扩大检索关键词(如RNF144A，可能的别名)进一步查询。
Recombinant human IBRDC1 (Interleukin-1 Receptor Associated Kinase 1-Binding Protein 1) is a protein of interest in cellular signaling and immune regulation. IBRDC1. also known as IRAK1BP1. contains an N-terminal serine-rich domain and a C-terminal ubiquitin-like fold. It interacts with interleukin-1 receptor-associated kinase 1 (IRAK1), a critical mediator in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. By modulating IRAK1 activity, IBRDC1 influences NF-κB activation, cytokine production, and inflammatory responses. Studies suggest it acts as a negative regulator, promoting IRAK1 degradation via the ubiquitin-proteasome system, thereby attenuating excessive immune activation. Dysregulation of IBRDC1 has been linked to autoimmune diseases and chronic inflammation. Recombinant IBRDC1 protein is produced using expression systems (e.g., E. coli, mammalian cells) for functional studies, including protein-protein interaction assays, ubiquitination analyses, and drug screening. Its structural and functional characterization aids in understanding innate immunity mechanisms and developing therapeutic strategies targeting inflammatory disorders.
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