| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HYPE |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-449aa |
| 氨基酸序列 | MAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP |
| 分子量 | 75.13 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人HYPE蛋白(FICD/AMPylase)的参考文献示例(文献标题与内容根据公开研究综合整理，可能存在虚构，建议核对原始文献)：
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1. **"Structural insights into the AMPylation activity of human FICD/HYPE"**
*作者：Smith J. et al. (2020)*
**摘要**：通过X射线晶体学解析了重组人HYPE蛋白的催化结构域，揭示了其AMPylation(Fic介导的翻译后修饰)的分子机制，发现ATP结合口袋的关键残基突变显著影响其对底物(如BiP/GRP78)的修饰能力。
2. **"Recombinant HYPE protein regulates ER stress through AMPylation of molecular chaperones"**
*作者：Chen L. et al. (2018)*
**摘要**：研究利用重组HYPE蛋白在体外验证其对内质网分子伴侣BiP的AMPylation作用，证明其通过动态调控BiP活性参与内质网未折叠蛋白反应(UPR)，影响细胞应激应答通路。
3. **"Functional characterization of FICD/HYPE variants in neurodegenerative disorders"**
*作者：Kim S. et al. (2021)*
**摘要**：通过表达纯化多种HYPE突变体，发现某些与阿尔茨海默病相关的错义突变导致AMPylation活性异常，提示HYPE功能紊乱可能与蛋白质错误折叠疾病相关。
4. **"Development of a high-throughput assay for FICD-mediated AMPylation using recombinant protein technology"**
*作者：Wang Y. et al. (2019)*
**摘要**：报道了一种基于重组HYPE蛋白的荧光检测方法，用于快速筛选AMPylation抑制剂或激活剂，为靶向HYPE的药物开发提供工具。
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**注意**：以上文献为示例，部分内容可能整合自真实研究(如HYPE与BiP的相互作用)，但具体标题与作者需通过PubMed/Google Scholar等平台使用关键词 **"HYPE"、"FICD"、"AMPylation"** 检索核实。
Recombinant human HYPE (Huntingtin Yeast Partner E) protein is a engineered variant of the naturally occurring HYPE enzyme, which belongs to the fic protein family. Originally identified as a binding partner of huntingtin, the protein mutated in Huntington's disease, HYPE functions as an AMPylation enzyme that post-translationally modifies target proteins by transferring AMP molecules to threonine residues. This modification regulates various cellular processes, including the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress pathways.
The recombinant form is typically expressed in Escherichia coli or mammalian cell systems, enabling controlled studies of its structure-function relationships and enzymatic activity. Researchers utilize recombinant HYPE to investigate its role in proteostasis, particularly its interaction with key chaperones like BiP/GRP78 in ER stress management. Its AMPylation activity has implications in neurodegenerative diseases, cancer, and microbial pathogenesis, making it a focal point for therapeutic exploration.
Recent studies highlight HYPE's dual role as both a stress response modulator and a potential pathogenic factor when dysregulated. Production of recombinant HYPE allows for high-purity enzyme supplies needed for biochemical assays, inhibitor screening, and structural biology (e.g., crystallography). Ongoing research aims to unravel its substrate specificity and develop small-molecule modulators targeting AMPylation-dependent cellular mechanisms.
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