| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTRA3 |
| Uniprot No | P83110 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MQARALLLAALAALALAREPPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGACPLGLHQLSSPRYKFNFIADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIITNAHVVSSNSAAPGRQQLKVQLQNGDSYEATIKDIDKKSDIATIKIHPKKKLPVLLLGHSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQREGRELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRITRFLTEFQDKQIKDWKKRFIGIRMRTITPSLVDELKASNPDFPEVSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLTESPLLLEVRRGNDDLLFSIAPEVVM |
| 分子量 | 76.23 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人HTRA3蛋白的参考文献及其摘要内容的简要概括：
1. **文献名称**：*"Characterization of Recombinant Human HTRA3 and Its Role in Trophoblast Cell Function"*
**作者**：Singh H et al.
**摘要内容**：研究利用大肠杆菌系统表达并纯化重组人HTRA3蛋白，验证其丝氨酸蛋白酶活性，发现其通过调控TGF-β信号通路影响胎盘滋养层细胞的迁移与侵袭，可能与先兆子痫的发病机制相关。
2. **文献名称**：*"Structural and Functional Analysis of HTRA3 Protease Domain"*
**作者**：Li Y et al.
**摘要内容**：解析重组HTRA3蛋白催化结构域的晶体结构，揭示其底物结合位点特征，并通过体外实验证明其对细胞外基质蛋白(如纤连蛋白)的降解活性，提示其在组织重塑中的作用。
3. **文献名称**：*"Recombinant HTRA3 Inhibits Ovarian Cancer Cell Proliferation via EGFR Signaling Suppression"*
**作者**：Wang Q et al.
**摘要内容**：在卵巢癌细胞系中，外源添加重组HTRA3蛋白可通过抑制EGFR磷酸化阻断下游MAPK/ERK通路，显著降低癌细胞增殖能力，表明其作为肿瘤抑制因子的潜力。
4. **文献名称**：*"HTRA3 in Embryonic Development: Insights from Recombinant Protein-Based Models"*
**作者**：Gao R et al.
**摘要内容**：利用重组HTRA3蛋白处理胚胎干细胞，发现其通过诱导Smad2/3蛋白降解调控胚胎早期分化和凋亡，强调其在哺乳动物发育中的关键作用。
注：上述文献信息为概括性示例，实际文献名称与作者可能需要根据具体研究调整。建议通过PubMed或Web of Science以关键词“recombinant HTRA3”或“HTRA3 protein”检索最新文献。
Human HTRA3 (High-temperature requirement A3) is a serine protease belonging to the conserved HTRA protein family, characterized by a trypsin-like protease domain and a PDZ regulatory domain. It plays multifaceted roles in cellular processes, including extracellular matrix remodeling, apoptosis, and embryonic development. In humans, HTRA3 exists in two isoforms: a full-length intracellular form and a truncated secretory form generated by alternative splicing. The protein is highly expressed in placental tissues, where it regulates trophoblast invasion and spiral artery remodeling during pregnancy, with dysregulation linked to preeclampsia and miscarriage. It also exhibits context-dependent roles in cancer, acting as either a tumor suppressor by degrading oncogenic substrates or promoting metastasis in certain microenvironments. Recombinant human HTRA3 protein, typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, enables functional studies of its protease activity, substrate interactions, and therapeutic potential. Its structural features—particularly the catalytic triad (His, Asp, Ser) and PDZ domain-mediated substrate recognition—make it a target for drug development in pregnancy complications and malignancies. Current research focuses on elucidating its regulatory networks, including interactions with TGF-β signaling pathways and matrix metalloproteinases.
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