| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR3B |
| Uniprot No | O95264 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MLSSVMAPLWACILVAAGILATDTHHPQDSALYHLSKQLLQKYHKEVRPVYNWTKATTVYLDLFVHAILDVDAENQILKTSVWYQEVWNDEFLSWNSSMFDEIREISLPLSAIWAPDIIINEFVDIERYPDLPYVYVNSSGTIENYKPIQVVSACSLETYAFPFDVQNCSLTFKSILHTVEDVDLAFLRSPEDIQHDKKAFLNDSEWELLSVSSTYSILQSSAGGFAQIQFNVVMRRHPLVYVVSLLIPSIFLMLVDLGSFYLPPNCRARIVFKTSVLVGYTVFRVNMSNQVPRSVGSTPLIGHFFTICMAFLVLSLAKSIVLVKFLHDEQRGGQEQPFLCLRGDTDADRPRVEPRAQRAVVTESSLYGEHLAQPGTLKEVWSQLQSISNYLQTQDQTDQQEAEWLVLLSRFDRLLFQSYLFMLGIYTITLCSLWALWGGV |
| 分子量 | 50.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HTR3B蛋白的3条参考文献的概括示例：
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1. **文献名称**：*Structural insights into the human 5-HT3B receptor subunit and its role in ligand binding*
**作者**：Thompson AJ 等人
**摘要**：通过冷冻电镜技术解析了重组人HTR3B蛋白与HTR3A亚基共同形成的5-HT3受体异源二聚体结构，揭示了HTR3B在配体结合中的作用，并发现其与HTR3A的药理学差异。
2. **文献名称**：*Functional characterization of recombinant human HTR3B subunits in Xenopus oocytes*
**作者**：Davies PA 等人
**摘要**：研究在非洲爪蟾卵母细胞中表达重组人HTR3B蛋白，证明HTR3B单独无法形成功能性离子通道，需与HTR3A共表达以调节受体脱敏和电流幅度。
3. **文献名称**：*Expression and purification of recombinant human HTR3B for antipsychotic drug screening*
**作者**：Zhang Y 等人
**摘要**：开发了一种在HEK293细胞中高纯度表达重组人HTR3B蛋白的方法，并基于该蛋白建立高通量药物筛选平台，用于发现靶向5-HT3受体的新型抗精神病候选分子。
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*注：以上文献为示例性质，实际引用时需核实具体来源及细节。*
The human HTR3B gene encodes the 5-hydroxytryptamine receptor 3B (5-HT3B) subunit, a critical component of the serotonin-gated ion channel family. As a ligand-gated cation channel, the 5-HT3 receptor mediates fast synaptic transmission in the central and peripheral nervous systems. Unlike other serotonin receptors, which are G protein-coupled, the 5-HT3 receptor forms a pentameric structure. The functional receptor typically assembles as a heteromeric complex of HTR3A and HTR3B subunits, though HTR3A alone can form homomeric channels. The inclusion of HTR3B alters channel properties, enhancing ionic conductance and influencing receptor pharmacology.
Recombinant human HTR3B protein is produced via heterologous expression systems (e.g., mammalian or insect cells) for structural and functional studies. It has been pivotal in characterizing receptor-ligand interactions, ion permeation mechanisms, and subunit stoichiometry. Dysregulation of 5-HT3 receptors is linked to disorders like irritable bowel syndrome, chemotherapy-induced nausea, psychiatric conditions, and neuropathic pain. Pharmaceutical agents targeting 5-HT3 receptors, such as antiemetics (e.g., ondansetron), often require understanding HTR3B’s role in subtype-specific drug responses. Recent cryo-EM studies using recombinant HTR3B-containing receptors have provided atomic-level insights into channel gating and allosteric modulation. Current research focuses on developing subtype-selective compounds to minimize side effects and exploring HTR3B polymorphisms affecting drug efficacy. Its study bridges neurobiology, structural biology, and precision medicine.
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