| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR3A |
| Uniprot No | P46098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-478aa |
| 氨基酸序列 | RRSRNTTRPALLRLSDYLLTNYRKGVRPVRDWRKPTTVSIDVIVYAILNVDEKNQVLTTYIWYRQYWTDEFLQWNPEDFDNITKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYIRHQGEVQNYKPLQVVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINISLWRLPEKVKSDRSVFMNQGEWELLGVLPYFREFSMESSNYYAEMKFYVVIRRRPLFYVVSLLLPSIFLMVMDIVGFYLPPNSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQDLQQPVPAWLRHLVLERIAWLLCLREQSTSQRPPATSQATKTDDCSAMGNHCSHMGGPQDFEKSPRDRCSPPPPPREASLAVCGLLQELSSIRQFLEKRDEIREVARDWLRVGSVLDKLLFHIYLLAVLAYSITLVMLWSIWQYA |
| 分子量 | 59.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HTTR3A蛋白的3-4篇参考文献示例(内容基于常见研究方向，部分信息可能有虚构)：
---
1. **文献名称**：*Expression and pharmacological characterization of the human 5-HT3A receptor in a recombinant mammalian cell system*
**作者**：Smith, J., et al.
**摘要**：研究通过哺乳动物细胞(HEK293)系统成功表达了重组人HTR3A受体，并利用放射性配体结合实验和电生理记录验证了其功能活性，揭示了该受体对经典激动剂(如5-HT)和拮抗剂(如昂丹司琼)的敏感性。
2. **文献名称**：*Cryo-EM structure of the human 5-HT3A receptor in complex with a nanobody*
**作者**：Thompson, R., et al.
**摘要**：通过冷冻电镜技术解析了重组人HTR3A受体的高分辨率结构，揭示了其与特定纳米抗体结合的构象变化，为靶向该受体的药物设计提供了结构基础。
3. **文献名称**：*Functional modulation of recombinant human 5-HT3A receptors by intracellular signaling pathways*
**作者**：Lee, H., et al.
**摘要**：利用重组表达的HTR3A蛋白，研究了蛋白激酶C(PKC)和钙调蛋白对其离子通道功能的调控作用，表明受体活性可能受细胞内信号通路的动态调节。
4. **文献名称**：*High-yield production of recombinant human HTR3A extracellular domain for antibody development*
**作者**：Garcia, M., et al.
**摘要**：优化了HTR3A受体胞外结构域的重组表达方案(大肠杆菌系统)，并证明纯化蛋白可用于筛选特异性抗体，为相关诊断工具开发奠定了基础。
---
注：上述文献为示例，实际研究需参考真实发表的论文数据库(如PubMed)。
**Background of Recombinant Human HTR3A Protein**
The HTR3A gene encodes the 5-hydroxytryptamine (serotonin) receptor 3A subunit, a ligand-gated ion channel primarily involved in neurotransmission and gastrointestinal function. As a member of the Cys-loop receptor family, HTR3A forms homopentameric or heteromeric complexes (with HTR3B) to mediate rapid depolarization upon serotonin binding, influencing processes like nausea, vomiting, and gut motility. Dysregulation of HTR3 signaling is linked to disorders such as irritable bowel syndrome (IBS), chemotherapy-induced emesis, and neuropsychiatric conditions.
Recombinant human HTR3A protein is produced using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional ion channel assembly. This engineered protein facilitates structural studies (e.g., cryo-EM), ligand-binding assays, and drug discovery targeting HTR3A-related pathologies. Its applications extend to screening antagonists like ondansetron, a widely used antiemetic, and exploring novel therapeutics for anxiety or substance abuse.
Research on recombinant HTR3A also addresses challenges in receptor stoichiometry, allosteric modulation, and interspecies variability, providing insights into subtype-specific drug design. As a tool, it bridges molecular pharmacology and clinical translation, enhancing understanding of serotonin signaling in health and disease.
×
