关键信息
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基因名
JCHAIN
- 应用
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别名
JCHAIN；IGCJ；IGJ；Immunoglobulin J chain
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P01591
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表达区间
23-159aa
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氨基酸序列
QEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD
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分子量
28.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
JCHAIN, a crucial component of the immune system, encodes the J-chain protein that plays a significant role in the formation and stability of polymeric immunoglobulins, particularly IgM and IgA. Its primary function is to facilitate the secretion of these antibodies by linking monomeric immunoglobulins into larger structures, which are essential for effective immune responses. The understanding of JCHAIN's role has been pivotal in immunology, given that IgM and IgA antibodies are the first line of defense against pathogens. Recent studies have focused on the structural characterization and functional analysis of JCHAIN, employing advanced techniques such as X-ray crystallography and NMR spectroscopy to elucidate its mechanisms of action. These investigations provide insights into the molecular dynamics of immunoglobulin assembly and secretion, highlighting JCHAIN's impact on antibody functionality and immune system efficiency. Moreover, dysregulation in JCHAIN expression has been associated with various immune disorders and pathologies, making it a critical target for therapeutic intervention. Ongoing research aims to further dissect the regulatory pathways governing JCHAIN expression, its interactions with other immunological components, and its potential applications in vaccine development and treatment of autoimmune diseases. Understanding the multifaceted roles of JCHAIN not only enhances our knowledge of immune system dynamics but also opens new avenues for innovative strategies in immunotherapy and disease management.
