| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRPA1 |
| Uniprot No | P09651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-354aa |
| 氨基酸序列 | SKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQ |
| 分子量 | 40.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HNRPA1(HNRNPA1)蛋白的参考文献示例，部分基于真实研究改编，内容仅供参考：
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1. **文献名称**: *Expression and purification of recombinant human hnRNP A1 for structural and functional studies*
**作者**: Smith, J., et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达和纯化重组HNRNPA1蛋白的方法，并验证了其与单链RNA结合的功能活性，为后续结构解析提供了基础。
2. **文献名称**: *HNRNPA1 mutations cause familial amyotrophic lateral sclerosis via altered phase separation*
**作者**: Kim, H.J., et al. (2013. *Nature*)
**摘要**: 该研究发现HNRNPA1基因突变通过影响蛋白液液相分离(LLPS)参与肌萎缩侧索硬化症(ALS)的发病机制，并利用重组蛋白模型验证了突变体的异常聚集行为。
3. **文献名称**: *Recombinant HNRNPA1 regulates alternative splicing in cancer cells*
**作者**: Wang, L., et al.
**摘要**: 通过体外重建实验，证明重组HNRNPA1蛋白在癌细胞中调控特定mRNA的选择性剪接，揭示了其在肿瘤进展中的潜在作用。
4. **文献名称**: *Biophysical characterization of HNRNPA1-RNA interactions using surface plasmon resonance*
**作者**: Gonzalez, M., et al.
**摘要**: 利用表面等离子体共振技术(SPR)分析重组HNRNPA1蛋白与不同RNA序列的结合动力学，阐明了其RNA识别机制。
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**说明**：上述文献为领域内典型研究方向示例(如蛋白表达、疾病机制、互作研究)。具体研究请参考PubMed或Google Scholar最新文献，关键词“recombinant HNRNPA1”或“HNRNPA1 protein expression”。
**Background of Recombinant Human HNRNPA1 Protein**
HNRNPA1 (heterogeneous nuclear ribonucleoprotein A1) is a key RNA-binding protein involved in multiple steps of RNA metabolism, including pre-mRNA splicing, mRNA transport, stability, and translation regulation. It belongs to the hnRNP family and plays critical roles in cellular processes such as telomere maintenance, gene expression control, and stress response. Structurally, HNRNPA1 contains RNA recognition motifs (RRMs) and a glycine-rich domain, enabling interactions with nucleic acids and proteins.
Dysregulation of HNRNPA1 is linked to neurodegenerative diseases (e.g., amyotrophic lateral sclerosis, frontotemporal dementia) and cancers, often due to mutations promoting abnormal protein aggregation or altered RNA processing. Recombinant human HNRNPA1 protein, produced via expression systems like *E. coli* or mammalian cells, serves as a vital tool for studying RNA-protein interactions, splicing mechanisms, and disease pathogenesis. Its high purity and activity enable applications in *in vitro* assays, structural studies, and screening for therapeutic agents targeting RNA-related disorders. Research on HNRNPA1 also explores its role in stress granule dynamics, offering insights into cellular adaptation to environmental stresses.
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