| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPH3 |
| Uniprot No | P31942 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MDWVMKHNGPNDASDGTVRLRGLPFGCSKEEIVQFFQGLEIVPNGITLTMDYQGRSTGEAFVQFASKEIAENALGKHKERIGHRYIEIFRSSRSEIKGFYDPPRRLLGQRPGPYDRPIGGRGGYYGAGRGSYGGFDDYGGYNNYGYGNDGFDDRMRDGRGMGGHGYGGAGDASSGFHGGHFVHMRGLPFRATENDIANFFSPLNPIRVHIDIGADGRATGEADVEFVTHEDAVAAMSKDKNNMQHRYIELFLNSTPGGGSGMGGSGMGGYGRDGMDNQGGYGSVGRMGMGNNYSGGYGTPDGLGGYGRGGGGSGGYYGQGGMSGGGWRGMY |
| 分子量 | 61.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HNRNPH3蛋白的3-4篇参考文献示例(注：文献为虚拟示例，仅供格式参考)：
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1. **"Structural analysis of recombinant human HNRNPH3 reveals RNA-binding specificity"**
*Authors: Chen, X., Liu, Y., et al. (2020)*
**摘要**：本研究通过大肠杆菌系统表达并纯化了重组人HNRNPH3蛋白，结合X射线晶体学阐明了其RNA结合域的结构，揭示了其在pre-mRNA剪接中的特异性识别机制。
2. **"Recombinant HNRNPH3 modulates alternative splicing in glioblastoma cells"**
*Authors: Tanaka, M., Watanabe, R. (2019)*
**摘要**：利用哺乳动物细胞表达体系获得重组HNRNPH3.体外实验表明其通过调控EGFR基因的可变剪接促进胶质母细胞瘤的侵袭性。
3. **"Expression optimization and functional validation of HNRNPH3 in Drosophila models"**
*Authors: Gupta, S., Patel, K. (2021)*
**摘要**：开发了重组HNRNPH3蛋白的高效纯化方案，并在果蝇模型中验证了其通过RNA代谢通路调控神经发育的功能。
4. **"HNRNPH3 mutations disrupt RNA interaction in neurodevelopmental disorders"**
*Authors: Müller, F., et al. (2022)*
**摘要**：通过重组突变体蛋白的体外结合实验，证明HNRNPH3的Gly321突变导致其与靶RNA结合能力丧失，为相关神经发育异常提供分子机制解释。
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**说明**：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science以关键词“HNRNPH3 recombinant”“HNRNPH3 expression”等检索最新文献，或关注该蛋白在RNA代谢、癌症及神经系统疾病中的功能研究。
Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3) is a member of the HNRNP family, which plays critical roles in RNA metabolism, including splicing, transport, stability, and translation. HNRNPH3 specifically binds to RNA through its RNA recognition motifs (RRMs), facilitating interactions with guanine-rich sequences. This protein is ubiquitously expressed in human tissues and contributes to post-transcriptional regulation by modulating mRNA processing and export from the nucleus to the cytoplasm.
Recombinant HNRNPH3 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, RNA-binding specificity, and functional mechanisms *in vitro*. Research highlights its involvement in alternative splicing of pre-mRNAs linked to cellular proliferation and apoptosis, with dysregulation implicated in cancers (e.g., glioblastoma, colorectal cancer) and neurological disorders. Notably, HNRNPH3 interacts with other HNRNP proteins and cellular factors, forming dynamic complexes that adapt to transcriptional demands.
Studies using recombinant HNRNPH3 have advanced understanding of its role in diseases, offering potential as a diagnostic marker or therapeutic target. Its conserved domains and RNA-binding versatility make it a focal point for exploring gene expression regulation and developing RNA-targeted therapies.
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