| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRNPG-T |
| Uniprot No | O75526 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MVEADRPGKLFIGGFNLETDEKALEAEFGKYGRIVEVLLMKDRETNKSRGFAFVTFESPADAKAAARDMNGKSLDGKAIKVAQATKPAFESSRRGPPPPRSRGRPRFLRGTRGGGGGPRRSPSRGGPDDDGGYAADFDLRPSRAPMPMKRGPPPRRVGPPPKRAAPSGPARSSGGGMRGRALAVRGRDGYSGPPRREPLPPRRDPYLGPRDEGYSSRDGYSSRDYREPRGFAPSPGEYTHRDYGHSSVRDDCPLRGYSDRDGYGGRDRDYGDHLSRGSHREPFESYGELRGAAPGRGTPPSYGGGGRCEEYRGYSPDAYSGGRDSYSSSYGRSDRYSRGRHRVGRPDRGLSLSMERGCPPQRDSYSRSGCRVPRGGGRLGGRLERGGGRSRY |
| 分子量 | 69.52 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Functional characterization of the recombinant hnRNP G-T protein in alternative splicing regulation"**
- *Author(s): Zhang L, et al.*
- **摘要**：研究通过重组表达人HNRNPG-T蛋白，揭示其通过结合RNA增强特定外显子的选择性剪接，在癌症中表达异常可能与剪接失调相关。
2. **"Production and purification of recombinant HNRNPG-T for structural analysis"**
- *Author(s): Kim S, et al.*
- **摘要**：报道了在大肠杆菌系统中高效表达重组HNRNPG-T蛋白的方法，并利用质谱和X射线晶体学解析其结构，为功能研究提供基础。
3. **"HNRNPG-T interacts with oncogenic transcripts to promote tumor progression"**
- *Author(s): Patel R, et al.*
- **摘要**：发现重组HNRNPG-T在乳腺癌细胞中与促癌mRNA结合，调控其稳定性及翻译，敲低后抑制肿瘤生长，提示其作为治疗靶点潜力。
4. **"HNRNPG-T regulates viral RNA processing in host-pathogen interactions"**
- *Author(s): Gomez-Cardona EE, et al.*
- **摘要**：利用重组蛋白技术证实HNRNPG-T与HIV RNA结合，影响病毒剪接与包装，为抗病毒策略提供新方向。
*注：以上文献及摘要为模拟示例，实际研究需查询具体数据库。*
The heterogeneous nuclear ribonucleoprotein G-T (HNRNPG-T) is a splice variant of the HNRNPG gene, belonging to the hnRNP family involved in RNA metabolism. HNRNPG-T arises through alternative splicing or promoter usage, differing from other isoforms like HNRNPG. It contains RNA recognition motifs (RRMs) critical for binding pre-mRNA and regulating splicing, stability, and translation. HNRNPG-T is implicated in cancer progression, particularly in epithelial-mesenchymal transition (EMT), metastasis, and therapy resistance by modulating oncogenic transcripts. Its overexpression in tumors correlates with poor prognosis. Recombinant HNRNPG-T protein, produced via bacterial or mammalian expression systems with affinity tags (e.g., His-tag), enables functional studies to dissect molecular mechanisms. Researchers use it to explore RNA-protein interactions, splicing regulation, and therapeutic targeting. Its role in diseases beyond cancer, such as neurodegeneration, remains under investigation, highlighting its broad relevance in cellular RNA homeostasis.
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