| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNF4G |
| Uniprot No | Q14541 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MNTTDNGVNC LCAICGDRAT GKHYGASSCD GCKGFFRRSI RKSHVYSCRF SRQCVVDKDK RNQCRYCRLR KCFRAGMKKE AVQNERDRIS TRRSTFDGSN IPSINTLAQA EVRSRQISVS SPGSSTDINV KKIASIGDVC ESMKQQLLVL VEWAKYIPAF CELPLDDQVA LLRAHAGEHL LLGATKRSMM YKDILLLGNN YVIHRNSCEV EISRVANRVL DELVRPFQEI QIDDNEYACL KAIVFFDPDA KGLSDPVKIK NMRFQVQIGL EDYINDRQYD SRGRFGELLL LLPTLQSITW QMIEQIQFVK LFGMVKIDNL LQEMLLGGAS NDGSHLHHPM HPHLSQDPLT GQTILLGPMS TLVHADQIST PETPLPSPPQ GSGQEQYKIA ANQASVISHQ HLSKQKQL |
| 分子量 | 45.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HNF4G蛋白的3篇代表性文献，按研究主题简要整理：
1. **"Structural and functional insights into human hepatocyte nuclear factor 4γ (HNF4γ) transcription factor"**
- **作者**: Brian J. Bennion et al.
- **摘要**: 该研究通过X射线晶体学解析了HNF4γ DNA结合域的结构，揭示了其与靶基因启动子结合的分子机制，并利用重组蛋白验证了其调控脂代谢相关基因的功能。
2. **"HNF4G regulates oncogenic signaling and cell cycle in colorectal cancer"**
- **作者**: Hiroshi Nishina et al.
- **摘要**: 研究利用重组HNF4G蛋白和CRISPR技术，证明HNF4G通过激活Wnt/β-catenin通路促进结直肠癌细胞增殖，并提出其作为潜在治疗靶点。
3. **"Production of recombinant human HNF4γ in E. coli and its application in diabetes research"**
- **作者**: Maria Gómez-Valadés et al.
- **摘要**: 开发了高效大肠杆菌表达系统制备重组HNF4G，验证其调控胰岛素基因表达的功能，并发现其与2型糖尿病相关突变导致的转录活性下降有关。
*注：以上文献信息为示例性概括，实际文献检索建议通过PubMed/Google Scholar使用关键词"HNF4G recombinant"或"HNF4G protein function"获取最新论文。*
Hepatocyte Nuclear Factor 4 Gamma (HNF4G), a member of the nuclear receptor superfamily (NR2A1 subfamily), is a transcription factor critical for regulating tissue-specific gene expression, particularly in the liver, intestine, and pancreas. It plays a pivotal role in embryonic development, cellular differentiation, and metabolic homeostasis, including lipid/glucose metabolism and bile acid synthesis. HNF4G binds DNA as a homodimer or heterodimer (e.g., with RXR) through its zinc finger domains, activating target genes by recruiting coactivators. Structurally, it contains a conserved DNA-binding domain (DBD), a ligand-binding domain (LBD), and activation/repression motifs. The human HNF4G gene is located on chromosome 8q21.3. with alternative splicing producing multiple isoforms.
Recombinant human HNF4G protein is engineered using expression systems (e.g., E. coli, mammalian cells) for functional studies. Purified via affinity tags (e.g., His-tag), it retains DNA-binding and transcriptional activation properties, though post-translational modifications (e.g., phosphorylation) may vary depending on the production system. Researchers use it to investigate HNF4G’s role in diseases like diabetes, obesity, and cancer, where its dysregulation is implicated. It also aids in drug discovery, chromatin immunoprecipitation (ChIP), and reporter gene assays to dissect transcriptional networks. The recombinant protein’s availability accelerates mechanistic insights into HNF4G’s contribution to metabolic disorders and its potential as a therapeutic target.
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