| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIF3A |
| Uniprot No | Q9Y2N7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MALGLQRARS TTELRKEKSR DAARSRRSQE TEVLYQLAHT LPFARGVSAH LDKASIMRLT ISYLRMHRLC AAGEWNQVGA GGEPLDACYL KALEGFVMVL TAEGDMAYLS ENVSKHLGLS QLELIGHSIF DFIHPCDQEE LQDALTPQQT LSRRKVEAPT ERCFSLRMKS TLTSRGRTLN LKAATWKVLN CSGHMRAYKP PAQTSPAGSP DSEPPLQCLV LICEAIPHPG SLEPPLGRGA FLSRHSLDMK FTYCDDRIAE VAGYSPDDLI GCSAYEYIHA LDSDAVSKSI HTLLSKGQAV TGQYRFLARS GGYLWTQTQA TVVSGGRGPQ SESIVCVHFL ISQVEETGVV LSLEQTEQHS RRPIQRGAPS QKDTPNPGDS LDTPGPRILA FLHPPSLSEA ALAADPRRFC SPDLRRLLGP ILDGASVAAT PSTPLATRHP QSPLSADLPD ELPVGTENVH RLFTSGKDTE AVETDLDIAQ DADALDLEML APYISMDDDF QLNASEQLPR AYHRPLGAVP RPRARSFHGL SPPALEPSLL PRWGSDPRLS CSSPSRGDPS ASSPMAGARK RTLAQSSEDE DEGVELLGVR PPKRSPSPEH ENFLLFPLSL SFLLTGGPAP GSLQDPSTPL LNLNEPLGLG PSLLSPYSDE DTTQPGGPFQ PRAGSAQAD |
| 分子量 | 72.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人HIF3A蛋白的参考文献示例(注：以下内容为模拟生成，实际文献需查阅数据库验证)：
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1. **标题**：Expression and Functional Characterization of Recombinant Human HIF3α in Hypoxia Response
**作者**：Zhang et al. (2020)
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人HIF3α蛋白，证实其与缺氧反应元件(HRE)结合的能力，并通过细胞实验证明其可抑制HIF-1α介导的基因转录，提示其作为缺氧信号通路的负调控因子作用。
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2. **标题**：Structural Insights into HIF3α Isoforms and Their Interaction with ARNT
**作者**：Wang et al. (2019)
**摘要**：通过X射线晶体学解析重组人HIF3α蛋白的截短体结构，揭示了其与ARNT蛋白的异源二聚化机制，并发现特定结构域的缺失导致其对靶基因的抑制活性显著降低。
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3. **标题**：Recombinant HIF3α Protein Attenuates Ischemic Injury in a Mouse Model
**作者**：Li et al. (2021)
**摘要**：利用哺乳动物细胞系统表达重组人HIF3α蛋白，在小鼠缺血模型中验证其通过竞争性抑制HIF-1α/2α信号通路减轻组织损伤的效果，为治疗缺血性疾病提供潜在策略。
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建议通过PubMed或Web of Science搜索“recombinant human HIF3A protein”获取真实文献。
Hypoxia-inducible factor 3 alpha (HIF3A) is a subunit of the hypoxia-inducible factor (HIF) family, which plays a central role in cellular adaptation to low oxygen conditions. HIF complexes are heterodimers composed of an oxygen-sensitive α-subunit (HIF1A, HIF2A, or HIF3A) and a constitutively expressed β-subunit (ARNT). Unlike HIF1A and HIF2A, which activate hypoxia-responsive genes, HIF3A exhibits more complex regulatory roles. It lacks the C-terminal transactivation domain found in other α-subunits, suggesting it may act as a competitive inhibitor by forming nonfunctional heterodimers with ARNT or through alternative splice variants. Emerging studies indicate HIF3A's involvement in negative feedback loops to dampen hypoxic signaling, fine-tuning adaptive responses in tissues like the heart, lung, and placenta. However, its precise biological functions remain controversial, with evidence linking it to both tumor-suppressive and oncogenic processes depending on cellular context. Recombinant human HIF3A protein is commonly produced in E. coli or mammalian expression systems, enabling studies on its structure, DNA-binding properties, and interactions with regulatory proteins like prolyl hydroxylases and Von Hippel-Lindau tumor suppressor. Its applications span basic research in oxygen-sensing mechanisms, drug discovery for ischemic diseases, and cancer therapeutics.
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