| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H3F3B |
| Uniprot No | P84243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-136aa |
| 氨基酸序列 | ARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| 分子量 | 42.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人H3F3B蛋白的参考文献示例(注意：以下为虚构内容，仅作格式参考，实际文献需通过学术数据库检索):
1. **Title**: Expression and purification of recombinant human histone H3.3 (H3F3B) in E. coli
**Authors**: Zhang Y, et al.
**Summary**: 本文描述了一种在大肠杆菌中高效表达重组人H3F3B蛋白的方法，通过优化密码子和纯化流程获得高纯度蛋白，为核小体重建研究提供材料基础。
2. **Title**: Structural analysis of H3F3B mutations in pediatric glioblastoma
**Authors**: Chen L, et al.
**Summary**: 利用重组H3F3B蛋白解析了K36M致癌突变对组蛋白甲基化的影响，发现该突变通过抑制PRC2复合物活性驱动肿瘤发生。
3. **Title**: Chromatin assembly dynamics of recombinant H3F3B variants using single-molecule imaging
**Authors**: Smith RJ, et al.
**Summary**: 研究使用重组H3F3B蛋白结合荧光标记技术，揭示了H3.3变体在染色质组装过程中与CAF-1复合物的动态相互作用机制。
4. **Title**: Epigenetic profiling of H3F3B in induced pluripotent stem cells
**Authors**: Tanaka K, et al.
**Summary**: 通过体外重组H3F3B蛋白与DNA甲基转移酶的相互作用实验，证实H3.3在重编程过程中调控关键基因的表观遗传沉默。
*注：真实文献建议通过PubMed或Web of Science检索关键词 "H3F3B recombinant" "H3.3 variant" 等获取，可关注其在表观遗传、肿瘤生物学或染色质工程中的应用研究。*
The H3F3B gene encodes histone H3.3. a replication-independent variant of histone H3 that plays critical roles in chromatin structure and epigenetic regulation. Unlike canonical replication-dependent histones (e.g., H3.1/H3.2), H3.3 is incorporated into chromatin throughout the cell cycle, particularly at transcriptionally active regions, regulatory elements (promoters, enhancers), and sites like telomeres/centromeres. This dynamic deposition, mediated by chaperones like HIRA and DAXX/ATRX, allows H3.3 to influence gene expression, genome stability, and cellular differentiation.
Recombinant human H3F3B protein is produced via expression systems (e.g., E. coli, mammalian cells) to study its biochemical interactions, post-translational modifications (e.g., acetylation, methylation), and nucleosome assembly. It serves as a tool to explore how histone variants regulate chromatin dynamics in processes like DNA repair, transcriptional elongation, and cellular reprogramming. Dysregulation of H3F3B, including somatic mutations (e.g., K36M in chondroblastoma, G34W in giant cell tumors), is linked to cancer and developmental disorders. Recombinant H3F3B enables mechanistic studies of these mutations, aiding drug discovery and diagnostics. Its applications span structural biology, epigenetics, and disease modeling, offering insights into fundamental chromatin biology and therapeutic targeting of histone-related pathologies.
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