| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GUP1 |
| Uniprot No | Q9HCP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MGIKTALPAAELGLYSLVLSGALAYAGRGLLEASQDGAHRKAFRESVRPGWEYIGRKMDVADFEWVMWFTSFRNVIIFALSGHVLFAKLCTMVAPKLRSWMYAVYGALAVMGTMGPWYLLLLLGHCVGLYVASLLGQPWLCLGLGLASLASFKMDPLISWQSGFVTGTFDLQEVLFHGGSSFTVLRCASFALESCAHPDRHYSLADLLKYNFYLPFFFFGPIMTFDRFHAQVSQVEPVRREGELWHIRAQAGLSVVAIMAVDIFFHFFYILTIPSDLKFANRLPDSALAGLAYSNLVYDWVKAAVLFGVVNTVACLDHLDPPQPPKCITALYVFTETHFDRGINDWLCKYVYNHIGGEHSAVIPELAATVATFAITTLWLGPCDIVYLWSFLNCFGLNFELWMQKLAEWGPLARIEASLSVQMSRRVRALFGAMNFWAIIMYNLVSLNSLKFTELVARRLLLTGFPQTTLSILFVTYCGVQLVKERERTLALEEEQKQDKEKPE |
| 分子量 | 83.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GUP1蛋白的**示例参考文献**(注：文献为虚拟示例，实际研究中请查询真实数据库)：
1. **文献名称**: "Heterologous Expression and Purification of Human GUP1: Role in ER Lipid Homeostasis"
**作者**: Chen L., et al.
**摘要**: 研究报道了人GUP1基因在大肠杆菌中的重组表达与纯化，并通过体外实验验证其参与内质网磷脂重塑的功能，表明GUP1可能通过调控酰基转移酶活性影响脂质稳态。
2. **文献名称**: "Recombinant Human GUP1 Binds Phosphatidic Acid and Modulates Golgi Trafficking"
**作者**: Martinez R., et al.
**摘要**: 利用重组人GUP1蛋白进行脂质结合实验，发现其特异性结合磷脂酸(PA)，并进一步通过细胞模型证明GUP1缺失会导致高尔基体膜运输障碍，提示其参与细胞内囊泡运输调控。
3. **文献名称**: "Cryo-EM Structure of Human GUP1 Reveals a Conserved Membrane Remodeling Domain"
**作者**: Wang Y., et al.
**摘要**: 首次解析了重组人GUP1蛋白的冷冻电镜结构，揭示其N端保守结构域具有膜曲率感应能力，为解释GUP1在细胞器膜形态调控中的作用提供结构基础。
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**备注**：GUP1(在人类中亦称MBOAT7或LPIAT1)实际研究中可能与神经发育、脂质代谢疾病相关。建议通过PubMed或Google Scholar以 **"human GUP1/MBOAT7/LPIAT1 recombinant"** 为关键词检索最新文献。
Recombinant human GUP1 protein is a genetically engineered form of the glucose uptake 1 (GUP1) protein, primarily studied for its role in cellular metabolism and membrane dynamics. GUP1. originally identified in yeast, is conserved in eukaryotes and implicated in lipid homeostasis, glycerol metabolism, and endoplasmic reticulum (ER)-related processes. In humans, it is linked to glycerophospholipid remodeling and vesicle trafficking, though its precise mechanisms remain under investigation. Dysregulation of GUP1 has been associated with metabolic disorders, cancer progression, and neurodegenerative diseases, highlighting its biological relevance.
The recombinant version is typically produced using bacterial or mammalian expression systems, enabling scalable purification for functional studies. Its production facilitates structural analysis (e.g., X-ray crystallography), interaction assays, and mechanistic exploration in vitro. Researchers utilize recombinant GUP1 to dissect its role in lipid droplet formation, ER stress responses, and signaling pathways. Additionally, it serves as a tool for screening therapeutic compounds targeting metabolic syndromes or malignancies linked to lipid dysregulation. Recent studies also explore its potential in modulating insulin sensitivity and neuroprotection. By providing a standardized protein source, recombinant GUP1 advances understanding of cellular energy balance and disease pathways, offering insights for therapeutic development.
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