关键信息
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基因名
WWTR1
- 应用
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别名
WWTR1；TAZ；WW domain-containing transcription regulator Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9GZV5
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表达区间
1-400aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL
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分子量
47 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
WWTR1 (WW domain-containing transcription regulator 1), also known as TAZ (Transcriptional co-activator with PDZ-binding motif), is a key player in cellular signaling pathways, particularly in the Hippo signaling pathway, which regulates organ size and tissue homeostasis. Aberrant expression or mutations in WWTR1 have been implicated in various cancers and fibrotic diseases, signaling the importance of its role in cell proliferation and differentiation. Researchers have focused on the characterization of WWTR1 as a recombinant protein to elucidate its functional mechanisms and interactions with other cellular molecules. The recombinant form of WWTR1 provides a valuable tool for studying its structural properties, post-translational modifications, and binding affinities. By employing techniques such as X-ray crystallography and NMR spectroscopy, scientists aim to uncover the intricate details of WWTR1's role in transcriptional regulation and signal transduction. Additionally, understanding WWTR1's function could lead to novel therapeutic strategies for diseases related to its dysregulation, making it a significant focus of current biomedical research.
