| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLRA3 |
| Uniprot No | O75311 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-464aa |
| 氨基酸序列 | ARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDMDDEVRESRFSFTAYGMGPCLQAKDGMTPKGPNHPVQVMPKSPDEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVIYKILRHEDIHQQQD |
| 分子量 | 73.15 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的示例文献参考（非真实存在，仅供示例格式参考）：
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1. **文献名称**：Expression and Functional Characterization of Recombinant Human Glycine Receptor α3 Subunit in HEK293 Cells
**作者**：Smith J, et al.
**摘要**：本研究利用HEK293细胞成功表达了重组人GLRA3蛋白，并验证其形成功能性氯离子通道的能力。通过电生理实验证明，重组GLRA3对甘氨酸及士的宁敏感，为研究神经痛觉敏感化机制提供了工具。
2. **文献名称**：Structural Insights into GLRA3 Mutations Linked to Hyperekplexia via Cryo-EM Analysis
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析了重组GLRA3蛋白的三维结构，发现特定突变导致甘氨酸结合位点构象改变，阐明了其在过度惊厥症中的致病机理，为靶向药物设计提供结构基础。
3. **文献名称**：Development of a High-Yield Purification Protocol for Recombinant GLRA3 Extracellular Domain
**作者**：Wang Y, et al.
**摘要**：优化了重组GLRA3胞外段在昆虫细胞中的表达条件，采用亲和层析和凝胶过滤技术获得高纯度蛋白，适用于抗体开发及分子互作研究。
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**注意事项**：
- 以上文献信息为模拟示例，实际研究需通过PubMed、Web of Science等平台检索真实文献。
- 可尝试关键词：`"recombinant GLRA3"`、`"glycine receptor alpha3 expression"`、`"GLRA3 structure-function"`。
- 关注近年研究（如2020年后）以确保时效性。
The glycine receptor alpha 3 subunit (GLRA3) is a ligand-gated ion channel primarily expressed in the central nervous system, playing a critical role in inhibitory neurotransmission. As part of the glycine receptor family, GLRA3 forms pentameric chloride channels that mediate synaptic inhibition upon glycine binding, regulating neuronal excitability and motor coordination. Unlike other subunits (e.g., GLRA1), GLRA3 exhibits unique expression patterns, with higher abundance in hippocampal and cortical regions, suggesting specialized roles in cognitive functions and pain processing. Dysregulation of GLRA3 is implicated in neuropathological conditions, including hyperekplexia (startle disease), chronic pain, and epilepsy. Recombinant human GLRA3 protein is engineered using heterologous expression systems (e.g., HEK293 or insect cells) to study its structure-function relationships, ligand interactions, and disease-related mutations. Its production enables pharmacological screening for subtype-specific modulators, aiding the development of targeted therapies. Research on recombinant GLRA3 also clarifies its post-translational modifications, trafficking mechanisms, and roles in synaptic plasticity. Despite progress, GLRA3's full physiological impact remains underexplored, necessitating further studies to harness its therapeutic potential in neurological disorders.
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