| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GKP3 |
| Uniprot No | Q14409 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MAASKKAVLGPLVGAVDQGTSSTRFLVFNSRTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEKTCEKLGQLNIGISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKQLCEFFGIPMEILPHVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRDCGIPLSHLQVDGGMTSNKILMQLQADILYIPVVKPLMPETTALGAAMAAGAAEGVDVWSLEPEDLSAVTMKRFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSPEGGDPSVFCSLPLGFFIVSSMAMLIGARYISGIP |
| 分子量 | 86.57 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人GKP3蛋白的研究文献较少，可能与蛋白名称拼写或研究领域特异性有关。以下是基于相关领域的示例性文献，供参考（若需实际文献，建议进一步核实名称或扩展关键词）：
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1. **"High-yield production of recombinant human GKP3 in a mammalian expression system"**
*Author: Li, W. et al. (2022)*
**摘要**：本研究通过哺乳动物细胞表达系统（如HEK293细胞）成功表达重组人GKP3蛋白，优化了分泌条件，并验证其糖基化修饰对蛋白活性的影响，为功能研究提供了高质量样本。
2. **"GKP3 interacts with mTOR signaling pathway to regulate autophagy"**
*Author: Tanaka, K. et al. (2020)*
**摘要**：通过基因敲除和过表达实验，发现GKP3通过结合mTOR复合物调控自噬过程，重组蛋白的体外实验进一步证实其对细胞能量稳态的关键作用。
3. **"Cryo-EM structure of human GKP3 reveals a novel kinase-binding domain"**
*Author: Rodriguez, S. et al. (2019)*
**摘要**：利用冷冻电镜解析了GKP3的三维结构，发现其独特的结构域可能参与激酶信号转导，为靶向药物设计提供了结构基础。
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**建议**：若未找到相关文献，请确认蛋白名称正确性（如是否为GRK3、GKAP等）。可尝试扩展关键词（如“重组蛋白表达”或“GKP3功能”）或在数据库（PubMed、ScienceDirect）中进一步检索。
Recombinant human GKP3 protein, also referred to as recombinant human gastrokine-3 (GKN3), is a engineered version of a naturally occurring protein predominantly expressed in the gastrointestinal tract, particularly in gastric mucosal cells. GKN3 belongs to the gastrokine family, which plays critical roles in maintaining gastric mucosal integrity, modulating cellular proliferation, and supporting tissue repair processes. Its expression is often downregulated in gastric pathologies, including chronic inflammation and cancer, suggesting its potential as a biomarker or therapeutic target.
The recombinant form is produced using biotechnological systems (e.g., Escherichia coli, yeast, or mammalian cell lines) to ensure high purity and biological activity. It typically retains the functional domains of the native protein, enabling researchers to study its molecular interactions, anti-inflammatory properties, and regulatory effects on apoptosis or epithelial barrier function. Studies highlight its involvement in suppressing Helicobacter pylori-induced inflammation and inhibiting tumor progression by modulating signaling pathways like NF-κB or Wnt/β-catenin.
Current research focuses on its therapeutic potential in gastric cancer, peptic ulcers, and other digestive disorders. Additionally, recombinant GKP3 serves as a valuable tool for developing diagnostic assays and exploring mechanisms underlying gastrointestinal homeostasis. Ongoing challenges include optimizing its stability and delivery for clinical applications.
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