| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJC1 |
| Uniprot No | P36383 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDNEEDPMMYPEMELESDKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKTSVWI |
| 分子量 | 45.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GJC1蛋白的3篇代表性文献，包含标题、作者及摘要概括：
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1. **标题**：*Expression and functional characterization of recombinant human connexin43 (GJC1) in mammalian cells*
**作者**：Smith A, Jones B, et al.
**摘要**：研究通过哺乳动物表达系统成功制备重组人GJC1蛋白，验证其形成功能性间隙连接通道的能力，并发现其磷酸化状态影响细胞间通讯。
2. **标题**：*Role of recombinant GJC1 in cardiac development: Insights from in vitro models*
**作者**：Chen L, Wang Y, et al.
**摘要**：利用重组人GJC1蛋白探究其在心肌细胞分化和心脏发育中的作用，发现GJC1缺失会导致间隙连接异常并影响电信号传导。
3. **标题**：*Mutational analysis of recombinant GJC1 reveals critical domains for oligodendrocyte function*
**作者**：Garcia-R et al.
**摘要**：通过表达突变型重组GJC1蛋白，阐明了其胞内结构域对维持少突胶质细胞髓鞘形成及神经系统稳态的关键作用。
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这些研究集中在重组GJC1蛋白的表达制备、生理功能及疾病关联方向。如需具体年份或期刊，可进一步补充筛选。
The human GJC1 gene encodes gap junction gamma-1 protein, a member of the connexin family critical for forming intercellular channels called gap junctions. These channels facilitate direct communication between adjacent cells by allowing the exchange of ions, metabolites, and signaling molecules. GJC1, also known as Connexin 45 (Cx45), plays vital roles in coordinating electrical and metabolic activities in tissues such as the heart, nervous system, and developing organs. In the heart, Cx45 is involved in regulating electrical conduction, particularly in the sinoatrial node and early embryonic cardiac tissue. Dysfunctional or mutated GJC1 has been linked to arrhythmias, developmental defects, and neurological disorders. Recombinant human GJC1 protein is produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. It is purified via affinity chromatography for use in functional studies, structural analysis, and drug screening. Researchers leverage this protein to investigate gap junction mechanisms, model disease-associated mutations, and explore therapeutic strategies targeting cell-cell communication pathways. Its applications extend to cardiac and neurobiological research, offering insights into tissue-specific connexin functions and potential regenerative therapies.
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