| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJB7 |
| Uniprot No | Q6PEY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233aa |
| 氨基酸序列 | MSWMFLRDLLSGVNKYSTGTGWIWLAVVFVFRLLVYMVAAEHVWKDEQKEFECNSRQPGCKNVCFDDFFPISQVRLWALQLIMVSTPSLLVVLHVAYHEGREKRHRKKLYVSPGTMDGGLWYAYLISLIVKTGFEIGFLVLFYKLYDGFSVPYLIKCDLKPCPNTVDCFISKPTEKTIFILFLVITSCLCIVLNFIELSFLVLKCFIKCCLQKYLKKPQVLSVMSWMFLRDLLSGVNKYSTGTGWIWLAVVFVFRLLVYMVAAEHVWKDEQKEFECNSRQPGCKNVCFDDFFPISQVRLWALQLIMVSTPSLLVVLHVAYHEGREKRHRKKLYVSPGTMDGGLWYAYLISLIVKTGFEIGFLVLFYKLYDGFSVPYLIKCDLKPCPNTVDCFISKPTEKTIFILFLVITSCLCIVLNFIELSFLVLKCFIKCCLQKYLKKPQVLSV |
| 分子量 | 52.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的3篇关于重组人GJB7蛋白的模拟参考文献示例，供参考：
1. **文献名称**: *Expression and Purification of Recombinant Human GJB7 in E. coli*
**作者**: Smith A, et al.
**摘要**: 本研究成功构建了人GJB7蛋白的原核表达系统，优化了重组蛋白的可溶性表达及纯化流程，为后续功能研究提供了高纯度蛋白样品。
2. **文献名称**: *Functional Characterization of GJB7 in Intercellular Communication*
**作者**: Lee JH, Kim S.
**摘要**: 通过体外细胞模型证明重组人GJB7蛋白可形成功能性缝隙连接通道，调节细胞间离子和小分子物质的传递，提示其在组织稳态中的潜在作用。
3. **文献名称**: *Structural Insights into Human GJB7 by Cryo-EM*
**作者**: Wang Q, et al.
**摘要**: 采用冷冻电镜技术解析了重组人GJB7的高分辨率三维结构，揭示了其跨膜通道的关键氨基酸构成及可能的功能调控位点。
---
**注意**：以上文献为基于学术逻辑构造的模拟示例，实际研究需通过PubMed、Web of Science等数据库检索真实文献（关键词建议：*GJB7*/*Connexin 25* + *recombinant*/*knockout*/*function*）。
Gap junction beta-7 (GJB7), also known as connexin 25 or connexin 25.3, is a member of the connexin protein family that forms intercellular channels called gap junctions. These channels facilitate direct communication between adjacent cells by allowing the exchange of ions, metabolites, and signaling molecules. GJB7 is primarily expressed in the skin, inner ear, and other epithelial tissues, where it plays a role in cellular homeostasis and tissue-specific functions. While less studied compared to other connexins (e.g., GJB2, GJB6), emerging evidence suggests its involvement in dermatological and auditory pathologies. Mutations in the GJB7 gene have been tentatively linked to hereditary hearing loss and skin disorders, though its exact physiological and pathological mechanisms remain under investigation.
Recombinant human GJB7 protein is produced through genetic engineering, typically using bacterial or mammalian expression systems. It enables researchers to study GJB7's structure-function relationships, interaction partners, and regulatory pathways in vitro. This tool is critical for elucidating its role in cell signaling, tissue development, and disease mechanisms. Additionally, recombinant GJB7 serves as an antigen for antibody development or a substrate for drug screening targeting connexin-related disorders. Current challenges in its production include optimizing protein solubility and preserving post-translational modifications essential for native functionality. Further research on recombinant GJB7 could advance therapies for connexin-associated diseases and improve understanding of intercellular communication networks.
×
