| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJB4 |
| Uniprot No | Q9NTQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-266aa |
| 氨基酸序列 | MNWAFLQGLLSGVNKYSTVLSRIWLSVVFIFRVLVYVVAAEEVWDDEQKDFVCNTKQPGCPNVCYDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREERERKHHLKHGPNAPSLYDNLSKKRGGLWWTYLLSLIFKAAVDAGFLYIFHRLYKDYDMPRVVACSVEPCPHTVDCYISRPTEKKVFTYFMVTTAAICILLNLSEVFYLVGKRCMEIFGPRHRRPRCRECLPDTCPPYVLSQGGHPEDGNSVLMKAGSAPVDAGGYP |
| 分子量 | 55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人GJB4蛋白的参考文献概要，列举3篇示例：
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1. **文献名称**：*Functional characterization of recombinant human connexin 30.3 (GJB4) in epidermal barrier formation*
**作者**：Zhang Y, et al. (2021)
**摘要**：研究利用HEK293细胞表达重组人GJB4蛋白，发现其形成的间隙连接通道在表皮细胞间通讯中起关键作用，特定致病突变导致通道功能异常，与遗传性掌跖角化症（PPK）发病相关。
2. **文献名称**：*Expression and structural analysis of GJB4 mutations linked to skin disorders*
**作者**：Wang L, et al. (2020)
**摘要**：通过哺乳动物表达系统纯化重组GJB4蛋白，结合冷冻电镜解析其结构，揭示致病突变（如p.R22H）破坏蛋白寡聚化，影响角质细胞间通讯并诱导细胞凋亡。
3. **文献名称**：*Role of recombinant GJB4 in keratinocyte differentiation and wound healing*
**作者**：Li H, et al. (2022)
**摘要**：体外实验表明重组GJB4蛋白通过调控β-catenin信号通路促进角质形成细胞分化，突变体导致细胞迁移能力下降，为GJB4在皮肤屏障修复中的功能提供新证据。
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**注**：以上文献信息为基于GJB4研究的常规内容模拟生成，实际引用请以真实文献为准。建议通过PubMed或Web of Science以关键词“recombinant GJB4”“connexin 30.3”检索最新论文。
Recombinant human GJB4 protein is a genetically engineered form of the gap junction beta-4 protein (GJB4), also known as connexin 30.2 or connexin 31.3, which belongs to the connexin family of transmembrane proteins. These proteins assemble into gap junctions, intercellular channels facilitating direct communication between neighboring cells by allowing the passage of small molecules, ions, and signaling factors. GJB4 is particularly expressed in epithelial tissues and plays a critical role in maintaining skin homeostasis, epidermal differentiation, and auditory function. Mutations in the GJB4 gene have been linked to hereditary skin disorders such as erythrokeratodermia variabilis and hearing loss syndromes. The recombinant version is typically produced using bacterial or mammalian expression systems, enabling large-scale purification for research and therapeutic applications. Scientists utilize recombinant GJB4 to study its structure-function relationships, pathological mechanisms in connexin-related diseases, and potential therapeutic interventions. Recent studies also explore its involvement in cell proliferation regulation and wound healing processes. As a tool in dermatology and otology research, recombinant GJB4 helps advance drug discovery and gene therapy strategies targeting connexin-associated disorders.
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