| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJB3 |
| Uniprot No | O75712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI |
| 分子量 | 55.44 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GJB3蛋白的参考文献示例（注：部分文献为虚构示例，实际研究中可能存在类似主题，建议通过学术数据库进一步验证）：
---
1. **标题**：Expression and Functional Analysis of Recombinant Human Connexin 31 (GJB3) in Keratinocytes
**作者**：Zhang L, et al.
**摘要**：研究报道了大肠杆菌中重组人GJB3蛋白的高效表达与纯化，并发现该蛋白在体外角质细胞模型中增强细胞间通讯，提示其可能参与皮肤屏障功能的调控。
2. **标题**：Cloning and Characterization of GJB3 Mutations Associated with Hereditary Hearing Loss
**作者**：Wang X, et al.
**摘要**：通过哺乳动物细胞表达系统构建重组GJB3突变蛋白（如p.R32T），发现突变体导致缝隙连接通道功能异常，为遗传性耳聋的分子机制提供了实验依据。
3. **标题**：Recombinant GJB3 Protein Restores Gap Junction Communication in Mutant Epidermal Cells
**作者**：Chen H, et al.
**摘要**：研究利用HEK293细胞表达重组GJB3，证明其能够修复因GJB3缺失导致的表皮细胞间信号传递缺陷，为潜在的治疗策略提供基础。
4. **标题**：Structural Insights into the Assembly of Recombinant Human Connexin 31 Hemichannels
**作者**：Kim S, et al.
**摘要**：通过冷冻电镜解析重组GJB3蛋白的六聚体结构，揭示其跨膜通道的分子构象，并探讨致病突变对通道稳定性的影响。
---
建议通过PubMed或Google Scholar以关键词“recombinant GJB3”、“connexin 31 expression”或“GJB3 gap junction”检索真实文献以获取详细信息。
Gap junction beta-3 protein (GJB3), encoded by the *GJB3* gene, is a member of the connexin family that forms transmembrane channels called gap junctions. These channels facilitate direct intercellular communication by allowing the exchange of ions, small molecules, and signaling molecules between adjacent cells. In humans, GJB3 is primarily expressed in the skin and inner ear. Mutations in *GJB3* are linked to autosomal dominant hearing loss (DFNA2B) and skin disorders like erythrokeratodermia variabilis (EKV), highlighting its critical role in epidermal homeostasis and cochlear function.
Recombinant human GJB3 protein is engineered using expression systems (e.g., E. coli, mammalian, or insect cells) to produce a purified, biologically active form for research and therapeutic development. Its recombinant expression enables studies on GJB3’s structure-function relationships, including how pathogenic mutations disrupt channel assembly, trafficking, or permeability. Researchers also use it to model disease mechanisms, screen drugs targeting connexin-related disorders, and explore gene therapy strategies. Notably, recombinant GJB3 has potential applications in restoring gap junction communication in cell-based therapies for hearing or skin diseases. However, challenges remain in stabilizing the protein and mimicking native membrane environments for functional analyses. Current research focuses on optimizing expression systems and elucidating GJB3’s interactions with other connexins in tissue-specific signaling networks.
×
