| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJA8 |
| Uniprot No | P48165 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-433aa |
| 氨基酸序列 | MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLMYVGHAVHYVRMEEKRKSREAEELGQQAGTNGGPDQGSVKKSSGSKGTKKFRLEGTLLRTYICHIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKTIFILFMLSVASVSLFLNVMELGHLGLKGIRSALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKASSRARSDDLTV |
| 分子量 | 74.58 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GJA8蛋白的参考文献示例（基于公开研究主题，非真实文献数据，仅供格式参考）：
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1. **文献名称**：Expression and Functional Analysis of Recombinant Human GJA8 in Lens Epithelial Cells
**作者**：Smith A, et al.
**摘要**：研究通过哺乳动物表达系统成功重组表达人GJA8蛋白，发现其在细胞间通讯中的关键作用，并验证突变体GJA8导致晶状体钙信号紊乱，与先天性白内障相关。
2. **文献名称**：Structural Characterization of Human Connexin 50 (GJA8) Using Cryo-EM
**作者**：Chen L, Wang Y
**摘要**：利用冷冻电镜解析重组GJA8蛋白的跨膜通道结构，揭示了其六聚体组装模式及关键氨基酸对通道孔径的调控机制，为靶向药物设计提供结构基础。
3. **文献名称**：A Novel Mutation in GJA8 Causing Autosomal Dominant Congenital Cataract
**作者**：Zhang R, et al.
**摘要**：发现GJA8基因新发错义突变（p.R76H），通过重组蛋白体外实验证实该突变破坏间隙连接功能，导致细胞间代谢物转运异常，为临床白内障遗传机制提供证据。
4. **文献名称**：Recombinant GJA8 Channels Exhibit pH-Dependent Permeability in Vitro
**作者**：Johnson M, et al.
**摘要**：通过脂质体模型研究重组GJA8蛋白的离子通道特性，发现其通透性受pH值调控，低pH环境下通道关闭，可能参与晶状体氧化应激保护机制。
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（注：以上文献名为虚拟概括，实际文献需通过PubMed等数据库检索确认。）
Recombinant human GJA8 protein, also known as connexin 50 (Cx50), is a member of the connexin family of gap junction proteins. Encoded by the *GJA8* gene, it plays a critical role in cell-cell communication by forming intercellular channels that allow the passage of ions, metabolites, and signaling molecules. GJA8 is predominantly expressed in the lens of the eye, where it contributes to lens transparency and homeostasis. Mutations in *GJA8* are associated with congenital cataracts and other ocular disorders, highlighting its importance in maintaining lens integrity.
As a recombinant protein, GJA8 is produced using biotechnological methods, typically in *E. coli* or mammalian expression systems, to ensure proper folding and post-translational modifications. This engineered form enables researchers to study its structure-function relationships, channel gating mechanisms, and interactions with other lens proteins. Recombinant GJA8 serves as a valuable tool for investigating cataract pathogenesis, drug screening for therapeutic interventions, and developing diagnostic tools. Its role in gap junction-mediated signaling also extends to broader studies in cellular communication and tissue development. By leveraging recombinant GJA8, scientists aim to advance targeted therapies for eye diseases and explore its potential applications in regenerative medicine.
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