| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJA4 |
| Uniprot No | P35212 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLVYLGHVIYLSRREERLRQKEGELRALPAKDPQVERALAAVERQMAKISVAEDGRLRIRGALMGTYVASVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPYLVDCFVSRPTEKTIFIIFMLVVGLISLVLNLLELVHLLCRCLSRGMRARQGQDAPPTQGTSSDPYTDQVFFYLPVGQGPSSPPCPTYNGLSSSEQNWANLTTEERLASSRPPLFLDPPPQNGQKPPSRPSSSASKKQYV |
| 分子量 | 63.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GJA4蛋白的3篇示例参考文献（注：文献为模拟示例，实际引用需核实）：
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1. **文献名称**: "Expression and purification of recombinant human GJA4 in Escherichia coli for functional gap junction studies"
**作者**: Zhang, L. et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效表达重组人GJA4蛋白（Cx37），并优化了纯化流程，验证其形成功能性缝隙连接通道的能力。
2. **文献名称**: "Role of recombinant human GJA4 in vascular endothelial cell communication and atherosclerosis"
**作者**: Kumar, R. et al.
**摘要**: 利用哺乳动物细胞表达的重组GJA4蛋白，发现其调控血管内皮细胞间通讯，并影响动脉粥样硬化模型中斑块的形成。
3. **文献名称**: "Structural characterization of recombinant human Cx37 (GJA4) using cryo-electron microscopy"
**作者**: Chen, Y. et al.
**摘要**: 通过冷冻电镜解析了重组人GJA4蛋白的高分辨率结构，揭示了其通道开放的构象变化机制，为靶向治疗提供结构基础。
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**注意**：以上内容为模拟示例，实际文献需通过数据库（PubMed、Web of Science等）检索核实。如需具体文献推荐，请提供更详细的研究方向或应用场景。
Recombinant human GJA4 protein, also known as Connexin 37 (Cx37), is a synthetically produced variant of the gap junction protein encoded by the *GJA4* gene. Gap junction proteins form intercellular channels (connexons) that facilitate direct communication between adjacent cells by allowing the transfer of ions, metabolites, and signaling molecules. GJA4 is a transmembrane protein with four transmembrane domains, cytoplasmic N- and C-termini, and extracellular loops critical for channel docking. It is expressed in vascular endothelial cells, smooth muscle cells, and platelets, playing roles in vascular homeostasis, angiogenesis, and thromboregulation. Studies link GJA4 polymorphisms to cardiovascular diseases, including atherosclerosis and hypertension.
Recombinant GJA4 is typically expressed in *E. coli* or mammalian systems (e.g., HEK293 cells) for functional studies. Its production enables exploration of structure-function relationships, interactions with partners like Cx40 or Cx43, and mechanisms in cell proliferation/apoptosis. Researchers use it to model gap junction dysfunction in pathologies such as arrhythmias, aberrant clotting, or impaired vascular remodeling. Therapeutic applications focus on targeting connexins to modulate cell signaling in cancer metastasis or ischemic injury. Purified recombinant GJA4 is essential for antibody development, high-throughput drug screening, and structural analysis (e.g., cryo-EM) to advance precision therapies.
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