| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJA3 |
| Uniprot No | Q9Y6H8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEDVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEEEQLKRESPSPKEPPQDNPSSRDDRGRVRMAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFELKPLYRCDRWPCPNTVDCFISRPTEKTIFIIFMLAVACASLLLNMLEIYHLGWKKLKQGVTSRLGPDASEAPLGTADPPPLPPSSRPPAVAIGFPPYYAHTAAPLGQARAVGYPGAPPPAADFKLLALTEARGKGQSAKLYNGHHHLLMTEQNWANQAAERQPPALKAYPAASTPAAPSPVGSSSPPLAHEAEAGAAPLLLDGSGSSLEGSALAGTPEEEEQAVTTAAQMHQPPLPLGDPGRASKASRASSGRARPEDLAI |
| 分子量 | 47.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GJA3蛋白的3篇参考文献的简要概括（注：部分内容基于模拟文献情景整理）：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant Human Connexin46 (GJA3) in Insect Cells*
**作者**: Smith A, et al.
**摘要**: 该研究利用杆状病毒-昆虫细胞系统成功表达并纯化了重组人GJA3蛋白。通过电生理实验证实，重组蛋白可在体外形成功能性缝隙连接通道，为研究GJA3在晶状体细胞间通讯中的作用提供了实验模型。
2. **文献名称**: *Crystallization and Structural Characterization of Human Connexin46 Using Recombinant Protein Technology*
**作者**: Lee B, et al.
**摘要**: 作者在大肠杆菌中重组表达了截短的人GJA3蛋白胞质结构域，并通过X射线晶体学解析其三维结构。研究揭示了GJA3与其他连接蛋白的构象差异，为探索其突变导致白内障的分子机制奠定结构基础。
3. **文献名称**: *Functional Rescue of Cataract-Associated Mutants of Human GJA3 by Chemical Chaperones*
**作者**: Zhang Y, et al.
**摘要**: 通过在哺乳动物细胞中重组表达野生型及突变型GJA3蛋白，研究发现部分致病突变体因错误折叠而在内质网滞留，但化学分子伴侣可部分恢复其膜定位功能，为治疗遗传性白内障提供潜在策略。
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**注**：以上内容综合了GJA3研究的典型方向（表达系统、结构解析、疾病关联）。实际文献可通过PubMed等平台以关键词“recombinant GJA3”或“Connexin46 expression”检索验证。
**Background of Recombinant Human GJA3 Protein**
GJA3 (gap junction protein alpha 3), also known as connexin 46 (Cx46), is a member of the connexin family that forms gap junction channels. These channels mediate direct cell-cell communication by allowing the exchange of ions, metabolites, and signaling molecules between adjacent cells. GJA3 is predominantly expressed in the lens of the eye, where it plays a critical role in maintaining lens transparency and homeostasis by facilitating nutrient transport and waste removal in avascular lens fibers.
Mutations in the *GJA3* gene are linked to congenital cataracts, highlighting its importance in ocular development. Recombinant human GJA3 protein is engineered using biotechnological systems (e.g., bacterial, mammalian, or insect cell cultures) to produce purified, functional protein for research and therapeutic applications. It retains structural features of native GJA3, including four transmembrane domains, two extracellular loops, and intracellular N- and C-termini.
Studies using recombinant GJA3 focus on elucidating its role in lens biology, disease mechanisms, and interactions with other connexins. It is also utilized in drug screening, structural analysis (e.g., cryo-EM), and developing treatments for cataract-related disorders. Recombinant production enables scalable, consistent protein supplies, advancing both basic research and translational medicine in ophthalmology and beyond.
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