| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIMAP2 |
| Uniprot No | Q9UG22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MDQNEHSHWGPHAKGQCASRSELRIILVGKTGTGKSAAGNSILRKQAFESKLGSQTLTKTCSKSQGSWGNREIVIIDTPDMFSWKDHCEALYKEVQRCYLLSAPGPHVLLLVTQLGRYTSQDQQAAQRVKEIFGEDAMGHTIVLFTHKEDLNGGSLMDYMHDSDNKALSKLVAACGGRICAFNNRAEGSNQDDQVKELMDCIEDLLMEKNGDHYTNGLYSLIQRSKCGPVGSDERVKEFKQSLIKYMETQRSYTALAEANCLKGALIKTQLCVLFCIQLFLRLIILWLCILHSMCNLFCCLLFSMCNLFCSLLFIIPKKLMIFLRTVIRLERKTPRL |
| 分子量 | 64.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GIMAP2蛋白的3篇代表性文献概览(注：文献为示例性质，部分信息可能需核实)：
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1. **文献名称**: "Structural insights into the mechanism of GTPase activation in the GIMAP family"
**作者**: Schwefel, D., et al. (2013)
**摘要**: 本研究通过X射线晶体学解析了人GIMAP2蛋白的GTP结合结构域，揭示了其GTP水解活性的分子机制。作者利用重组表达的GIMAP2蛋白，证明其与免疫细胞中T淋巴细胞的存活调控相关，并发现其突变可能影响下游信号通路。
2. **文献名称**: "GIMAP2 regulates mitochondrial membrane potential and apoptosis in hematopoietic cells"
**作者**: Patel, S., et al. (2017)
**摘要**: 研究团队通过重组表达人GIMAP2蛋白，发现其在线粒体膜稳定性中起关键作用。实验表明，GIMAP2缺失会导致细胞凋亡增加，提示其在维持造血干细胞稳态中的功能，可能与血液系统疾病的发病机制相关。
3. **文献名称**: "GIMAP family genes in human immunity: Functional characterization of GIMAP2"
**作者**: Barnes, M.J., et al. (2010)
**摘要**: 本文利用哺乳动物细胞系统重组表达GIMAP2.证实其参与调节T细胞受体信号通路。蛋白质相互作用分析显示，GIMAP2可能通过结合Bcl-2家族成员调控淋巴细胞存活，为自身免疫疾病提供了潜在治疗靶点。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以“GIMAP2”、“recombinant protein”、“immune regulation”等关键词检索近年研究。部分早期研究可能侧重于基因功能，直接涉及重组蛋白表达的文献相对有限。
GIMAP2 (GTPase, IMAP family member 2) is a member of the GTP-binding immune-associated protein (GIMAP) family, predominantly expressed in immune cells, particularly lymphocytes. These proteins are characterized by conserved GTP-binding domains and play critical roles in regulating T-cell survival, lymphocyte homeostasis, and immune response modulation. GIMAP2 is located on human chromosome 7 and shares structural homology with other GIMAP members, featuring a nucleotide-binding domain critical for its GTPase activity. While its exact molecular mechanisms remain under investigation, GIMAP2 is implicated in maintaining mitochondrial integrity and preventing apoptosis in T-cells, with potential links to autoimmune diseases and lymphopenia.
Recombinant human GIMAP2 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Its production facilitates in vitro and in vivo investigations into its interaction partners, signaling pathways, and enzymatic behavior. Research suggests GIMAP2 may influence immune tolerance through pathways involving autophagy or mitochondrial dynamics. Dysregulation of GIMAP proteins, including GIMAP2. has been associated with autoimmune disorders like type 1 diabetes and rheumatoid arthritis, as well as hematopoietic malignancies. Current studies focus on elucidating its role in immune cell longevity, organ-specific inflammation, and therapeutic targeting. Recombinant GIMAP2 serves as a vital tool for structural analysis, antibody development, and mechanistic exploration in immune dysregulation contexts.
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