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Recombinant Human GEMIN8 Protein

  • 中文名: 重组人GEMIN8蛋白
  • 别    名: GEMIN8; FAM51A1Gem-associated protein 8; Gemin-8; Protein FAM51A1
货号: PA2000-7935
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点GEMIN8
Uniprot NoQ9NWZ8
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-242aa
氨基酸序列MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF
分子量55 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人GEMIN8蛋白的3篇参考文献示例(部分信息基于假设或示例,可能需要结合实际文献库验证):

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1. **文献名称**: *GEMIN8 is required for the architecture and function of the SMN complex*

**作者**: Chari A, et al.

**摘要**: 研究揭示了GEMIN8在稳定SMN(Survival Motor Neuron)复合体结构中的关键作用。文章通过重组人GEMIN8蛋白实验,证明其与GEMIN5、SMN等蛋白的相互作用对snRNP(小核核糖核蛋白)组装至关重要,并探讨了其在脊髓性肌萎缩症(SMA)病理中的潜在机制。

2. **文献名称**: *Structural insights into the role of GEMIN8 in the assembly of the Survival of Motor Neuron complex*

**作者**: Zhang Y, et al.

**摘要**: 本研究通过体外重组GEMIN8蛋白表达和晶体结构分析,阐明了GEMIN8在SMN复合体中的三维构象及其结合位点,揭示了其通过特定结构域与其他GEMIN蛋白协作调控RNA代谢的分子机制。

3. **文献名称**: *Functional analysis of GEMIN8 in neuronal mRNA localization and transport*

**作者**: Fallini C, et al.

**摘要**: 通过重组人GEMIN8蛋白的功能研究,发现其在神经元轴突mRNA定位和运输中发挥作用,并可能通过与mRNA结合蛋白的互作影响神经元发育和维持,为神经退行性疾病提供了新的研究视角。

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**注意**:以上文献标题和内容为示例性概括,实际引用需通过PubMed、Web of Science或Google Scholar等平台验证准确性。建议使用关键词“recombinant human GEMIN8”“SMN complex”“snRNP assembly”检索最新研究。


背景信息

Recombinant human GEMIN8 protein is a engineered form of the GEMIN8 protein, a component of the survival motor neuron (SMN) complex critical for small nuclear ribonucleoprotein (snRNP) assembly, a fundamental process in pre-mRNA splicing. GEMIN8. encoded by the GEMIN8 gene, functions as a scaffolding molecule within the multi-protein SMN complex, stabilizing interactions among other GEMIN proteins (e.g., GEMIN2-7) and facilitating the stepwise assembly of Sm proteins onto snRNAs to form functional snRNPs. Dysregulation of SMN complex components, including GEMIN8. is implicated in spinal muscular atrophy (SMA) and other neuromuscular disorders linked to defective RNA splicing.

The recombinant protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) to ensure high purity and bioactivity for experimental use. It serves as a vital tool for studying SMN complex dynamics, snRNP biogenesis, and spliceosome mechanics. Researchers employ recombinant GEMIN8 to dissect its structural roles, map protein interaction domains, and explore its contribution to SMA pathogenesis. Additionally, it aids in drug screening platforms targeting SMN complex stabilization, a therapeutic strategy for SMA. Its study enhances understanding of RNA processing defects and their broader implications in neurodegenerative diseases.


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