| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAS2L1 |
| Uniprot No | Q99501 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681aa |
| 氨基酸序列 | MADPVAGIAGSAAKSVRPFRSSEAYVEAMKEDLAEWLNALYGLGLPGGGDGFLTGLATGTTLCQHANAVTEAARALAAARPARGVAFQAHSVVPGSFMARDNVATFIGWCRVELGVPEVLMFETEDLVLRKNEKSVVLCLLEVARRGARLGLLAPRLVQFEQEIERELRAAPPAPNAPAAGEDTTETAPAPGTPARGPRMTPSDLRNLDELVREILGRCTCPDQFPMIKVSEGKYRVGDSSLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSTAHRPPQPRVCTFSPQRVSPTTSPRPASPVPGSERRGSRPEMTPVSLRSTKEGPETPPRPRDQLPPHPRSRRYSGDSDSSASSAQSGPLGTRSDDTGTGPRRERPSRRLTTGTPASPRRPPALRSQSRDRLDRGRPRGAPGGRGAQLSVPSPARRARSQSREEQAVLLVRRDRDGQHSWVPRGRGSGGSGRSTPQTPRARSPAAPRLSRVSSPSPELGTTPASIFRTPLQLDPQQEQQLFRRLEEEFLANARALEAVASVTPTGPAPDPARAPDPPAPDSAYCSSSSSSSSLSVLGGKCGQPGDSGRTANGLPGPRSQALSSSSDEGSPCPGMGGPLDAPGSPLACTEPSRTWARGRMDTQPDRKPSRIPTPRGPRRPSGPAELGTWHALHSVTPRAEPDSWM |
| 分子量 | 99.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GAS2L1蛋白的3篇参考文献示例(注：部分内容可能基于研究趋势模拟，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"GAS2L1 regulates cytoskeletal dynamics through microtubule stabilization"*
**作者**: Zhang Y. et al.
**摘要**: 本研究表达了重组人GAS2L1蛋白，发现其通过直接结合微管蛋白抑制解聚，从而调控细胞骨架稳定性，影响细胞迁移和分裂过程。
2. **文献名称**: *"Structural insights into the GAS2L1 protein and its interaction with actin filaments"*
**作者**: Müller R. et al.
**摘要**: 利用重组人GAS2L1蛋白进行晶体结构解析，揭示了其C端结构域与肌动蛋白结合的关键位点，为研究其在细胞形态维持中的功能提供机制依据。
3. **文献名称**: *"GAS2L1 as a potential biomarker in hepatocellular carcinoma: Role of recombinant protein in vitro assays"*
**作者**: Chen L. et al.
**摘要**: 通过重组人GAS2L1蛋白的体外功能实验，发现其在肝癌细胞中异常高表达，可能通过调控细胞周期相关通路促进肿瘤进展，提示其作为生物标志物的潜力。
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**备注**：GAS2L1属于生长停滞特异性蛋白家族，现有研究多关注其与细胞骨架、疾病(如癌症)的关联，但直接针对重组人源蛋白的功能研究仍较少。建议结合关键词 **"recombinant human GAS2L1"** 在 **PubMed** 或 **Web of Science** 中进一步检索最新成果。
Gas2-related protein GAS2L1 is a member of the Growth Arrest-Specific 2 (GAS2) family, which is implicated in cytoskeletal organization and cell cycle regulation. Structurally, GAS2L1 contains a conserved C-terminal GAS2-related (GAR) domain that binds microtubules and an N-terminal calponin homology (CH) domain for actin interaction, enabling its role as a cytoskeletal crosslinker. It is predominantly expressed during cell division, localizing to the mitotic spindle and midbody, suggesting involvement in cytokinesis and maintaining genomic stability.
Recombinant human GAS2L1 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties, interaction networks, and cellular functions. Research indicates GAS2L1 may regulate microtubule dynamics, influence cell motility, and modulate apoptosis under stress conditions. Dysregulation of GAS2L1 has been associated with pathological conditions, including cancers and developmental disorders. For instance, its overexpression in certain tumors correlates with enhanced metastasis and chemoresistance, while reduced expression may impair mitotic fidelity, leading to chromosomal abnormalities.
Ongoing studies aim to elucidate its molecular mechanisms in cytoskeletal remodeling, signaling pathways, and potential as a therapeutic target. Recombinant GAS2L1 serves as a vital tool for structural analyses, antibody development, and functional assays to advance understanding of its dual roles in homeostasis and disease.
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