| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAPVD1 |
| Uniprot No | Q14C86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-629aa |
| 氨基酸序列 | MQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPSQQEKLFGEKGSDRFRQKVQEMVESNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLGKFDKSCVAAFLDVVIGGRAVETPPLSSVNLLEGLSRTVVYITYSQLITLVNFMKSVMSGDQLREDRMALDNLLANLPPAKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRTNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMISENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESFLDWVAHLMIRMSGRSVPAPAHQASVLCPA |
| 分子量 | 95.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GAPVD1蛋白的3篇参考文献摘要，涵盖功能、疾病关联及结构研究：
1. **"GAPVD1 regulates Rab5- and Rab21-dependent endocytosis through its GTPase-activating activity"**
*作者：Zhang et al. (2015)*
**摘要**：本研究通过重组人GAPVD1蛋白的体外表达，揭示了其作为Rab5和Rab21的GTP酶激活蛋白(GAP)的作用，调控内吞囊泡的形成与运输，验证了其在细胞内吞途径中的关键功能。
2. **"Overexpression of GAPVD1 promotes ovarian cancer cell invasion via EGFR signaling"**
*作者：Smith et al. (2018)*
**摘要**：文章发现GAPVD1在卵巢癌中异常高表达，并通过重组蛋白实验证实其通过增强EGFR内吞和下游信号通路激活，促进肿瘤细胞的迁移和侵袭。
3. **"Structural basis of GAPVD1-mediated Rab GTPase regulation in vesicular trafficking"**
*作者：Brown et al. (2022)*
**摘要**：利用重组人GAPVD1蛋白进行X射线晶体学研究，解析其VPS9结构域与Rab5的结合模式，阐明了GAPVD1通过变构效应调控Rab蛋白活性的分子机制。
*注：以上文献为模拟示例，实际引用需依据具体研究内容检索数据库(如PubMed)。若需真实文献，建议以“GAPVD1 recombinant protein”等关键词检索最新论文。*
Recombinant human GAPVD1 (GTPase-activating protein and VPS9 domain-containing 1) is a multidomain protein involved in regulating intracellular trafficking and signaling pathways. GAPVD1 belongs to the GTPase-activating protein (GAP) family, which modulates small GTPases like Rab proteins, critical for vesicle transport, membrane fusion, and receptor recycling. Structurally, it contains a conserved N-terminal GAP domain that facilitates GTP hydrolysis in target Rab GTPases and a C-terminal VPS9 domain, which may act as a guanine nucleotide exchange factor (GEF) to activate specific Rabs. This dual functionality suggests a role in coordinating vesicle dynamics and cargo sorting.
GAPVD1 is implicated in endosomal trafficking, lysosomal function, and receptor internalization, including processes linked to growth factor signaling and immune response. Dysregulation of GAPVD1 has been associated with pathological conditions, such as cancer and neurological disorders, where aberrant Rab-mediated transport contributes to disease progression. Recombinant GAPVD1 protein, typically produced in Escherichia coli or mammalian expression systems, enables functional studies to dissect its biochemical interactions, enzymatic activity, and structural features. It serves as a tool for identifying binding partners, probing Rab activation/inactivation cycles, and screening therapeutic agents targeting trafficking-related diseases. The protein’s role in cellular homeostasis underscores its relevance in both basic research and translational applications.
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