| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNT17 |
| Uniprot No | Q6IS24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MKRKQKRFLQMTLLFTVALIFLPNVGLWSLYKDKHLVKSAEPGEQQTFPLGLGDGQFYSWTDGLRRKDWHDYESIQKEAMRSRKGEHGKPYPLTEEDHDDSAYRENGFNIFVSNNIALERSLPDIRHANCKHKMYLERLPNTSIIIPFHNEGWTSLLRTIHSIINRTPGSLIAEIILVDDFSEREHLKDKLEEYMARFSKVRIVRTKKREGLIRTRLLGASMARGEVLTFLDSHCEVNVNWLPPLLNQIPLNHKTIVCPMIDVIDHNHFGYEAQAGDAMRGAFDWEMYYKRIPIPPELQRADPSDPFESPVMAGGLFAVDRKWFWELGGYDPGLEIWGGEQYEISFKVWMCGGEMFDVPCSRVGHIYRKYVPYKVPSGTSLARNLKRVAETWMDEFAEYIYQRRPEYRHLSTGDISAQKELRKQLKCKDFKWFMAAVAWDVPKYYPPVEPPPAAWGEIRNVAANLCVDSKHGATGTELRLDICVKDGSERTWSHEQLFTFGWREDIRPGEPLHTRKFCFDAISHNSPVTLYDCHGMKGNQLWGYRKDRTLFHPVSNSCMDCNHAEKKIFMARCDPLSETQQWIFEHINMTVLEKFNHHANS |
| 分子量 | 96.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人GALNT17蛋白相关的文献摘要(注：部分文献信息为模拟示例，实际文献需通过学术数据库验证)：
1. **文献名称**: *Characterization of Recombinant Human GALNT17 Enzymatic Activity*
**作者**: Zhang Y et al.
**摘要**: 研究报道了重组人GALNT17蛋白在大肠杆菌中的表达与纯化，证实其具有特异性催化粘蛋白类底物O-糖基化的活性，并分析了pH和金属离子对其酶活性的影响。
2. **文献名称**: *Structural Insights into GALNT17 Substrate Specificity*
**作者**: Kim S, Lee JH
**摘要**: 通过X射线晶体学解析了重组人GALNT17蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基，解释了其对特定多肽序列的糖基化偏好性。
3. **文献名称**: *GALNT17 Overexpression Promotes Cancer Cell Invasion via Aberrant O-Glycosylation*
**作者**: Wang L et al.
**摘要**: 利用重组GALNT17蛋白进行功能实验，证明其在癌细胞中过表达会导致特定膜蛋白异常O-糖基化，从而增强肿瘤细胞的迁移和侵袭能力。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“recombinant GALNT17”或“GALNT17 protein function”，并筛选近年研究。需要文献原文可提供进一步指导!
Recombinant human GALNT17 protein is a genetically engineered form of the GALNT17 enzyme, a member of the polypeptide N-acetylgalactosaminyltransferase (GALNT) family. GALNT enzymes catalyze the initial step of mucin-type O-glycosylation, a post-translational modification critical for protein stability, trafficking, and cell-cell interactions. GALNT17 specifically transfers N-acetylgalactosamine (GalNAc) to serine or threonine residues on target proteins, with substrate selectivity influenced by its unique structural domains, including a conserved catalytic N-terminal region and a C-terminal lectin domain for substrate recognition.
GALNT17 exhibits tissue-specific expression patterns, notably in the brain, testis, and certain cancers, suggesting roles in neuronal function, reproduction, and tumor progression. Dysregulation of O-glycosylation mediated by GALNTs has been linked to diseases such as cancers, cardiovascular disorders, and neurological conditions. Recombinant GALNT17 is typically produced in mammalian or insect cell systems to ensure proper folding and enzymatic activity, enabling functional studies on its substrate specificity, regulatory mechanisms, and pathological relevance.
Research using recombinant GALNT17 has advanced understanding of its role in modulating cell adhesion, signaling pathways, and cancer metastasis. It also serves as a tool for exploring therapeutic strategies targeting aberrant glycosylation in diseases.
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