| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNAC4S-6ST |
| Uniprot No | Q7LFX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MRHCINCCIQLLPDGAHKQQVNCQGGPHHGHQACPTCKGENKILFRVDSKQMNLLAVLEVRTEGNENWGGFLRFKKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCWYEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQVLADEAFAWKTT |
| 分子量 | 87.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GALNAC4S-6ST蛋白的模拟参考文献示例(非真实文献，供格式参考)：
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1. **文献名称**：*Functional Expression and Characterization of Recombinant Human GalNAc4S-6ST in CHO Cells*
**作者**：Zhang L. et al.
**摘要**：本研究在CHO细胞中成功表达了重组人GALNAC4S-6ST蛋白，证实其在硫酸软骨素合成中催化4-硫酸基GalNAc残基的6-O-硫酸化作用，并通过质谱分析验证了其酶活性。
2. **文献名称**：*Role of GalNAc4S-6ST in Tumor Angiogenesis: Insights from Recombinant Protein Studies*
**作者**：Suzuki K. et al.
**摘要**：通过重组人GALNAC4S-6ST蛋白的功能实验，揭示了该酶通过修饰细胞表面糖链促进肿瘤血管生成，为癌症治疗提供了潜在靶点。
3. **文献名称**：*Structural Analysis of Recombinant GalNAc4S-6ST and Its Substrate Specificity*
**作者**：Wang H. et al.
**摘要**：解析了重组人GALNAC4S-6ST的晶体结构，阐明其底物结合域的关键氨基酸残基，揭示了其高效催化硫酸转移的分子机制。
4. **文献名称**：*GalNAc4S-6ST Knockout and Recombinant Protein Rescue in a Murine Model*
**作者**：Morimoto N. et al.
**摘要**：构建GALNAC4S-6ST基因敲除小鼠模型，通过外源重组蛋白回补实验证明该酶对胚胎发育中细胞外基质稳态的重要性。
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**备注**：以上文献为模拟示例，实际研究中请通过**PubMed/Google Scholar**检索关键词如 **"GalNAc4S-6ST", "CHST family sulfotransferase", "chondroitin sulfate sulfation"** 获取真实文献。
Recombinant human GALNAC4S-6ST (N-acetylgalactosamine-4-sulfate 6-O-sulfotransferase) is a key enzyme involved in the sulfation modifications of heparan sulfate proteoglycans (HSPGs), which play critical roles in cellular signaling, adhesion, and extracellular matrix organization. This enzyme catalyzes the transfer of sulfate groups to the C6 hydroxyl of N-acetylgalactosamine-4-sulfate (GalNAc4S) in glycosaminoglycan (GAG) chains, a step essential for generating structural diversity in HSPGs. Such sulfation patterns directly influence interactions with growth factors, chemokines, and pathogens, impacting processes like angiogenesis, inflammation, and viral entry.
The gene encoding GALNAC4S-6ST (officially named *CHST15*) has been linked to autoimmune disorders, cancer metastasis, and tissue fibrosis due to dysregulated sulfation. Recombinant forms of this protein are typically produced in mammalian expression systems to ensure proper post-translational modifications. These engineered proteins enable detailed studies of HSPG sulfation mechanisms and its biological implications.
Research utilizing recombinant GALNAC4S-6ST has advanced therapeutic development, including inhibitors targeting sulfotransferase activity to modulate pathological interactions in chronic inflammation or tumor microenvironments. Its role in fine-tuning HSPG-ligand specificity also makes it a tool for engineering biomaterials or targeted drug delivery systems. However, challenges remain in fully characterizing its substrate specificity and regulatory pathways, underscoring the need for further structural and functional investigations.
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