| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALK2 |
| Uniprot No | Q01415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIAVEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLEEMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICEEAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSFLANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA |
| 分子量 | 76.01 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GALK2蛋白的3条参考文献示例(注：以下内容为模拟虚构文献，实际引用请核实真实来源)：
1. **《Expression and functional characterization of recombinant human galactokinase 2 (GALK2) in E. coli》**
- 作者：Zhang, W. et al.
- 摘要：研究通过大肠杆菌表达系统成功制备了重组人GALK2蛋白，并验证其激酶活性。结果显示，GALK2对半乳糖的亲和力低于GALK1.但在调节细胞内半乳糖代谢中可能起补偿作用。
2. **《Structural insights into the catalytic mechanism of human GALK2 through X-ray crystallography》**
- 作者：Smith, J. & Lee, R.
- 摘要：通过X射线晶体学解析了重组人GALK2的三维结构，揭示了其ATP结合域和底物特异性机制，为靶向GALK2的药物设计提供结构基础。
3. **《Role of recombinant human GALK2 in modulating oxidative stress responses in cellular models》**
- 作者：Chen, L. et al.
- 摘要：研究发现重组人GALK2过表达可降低细胞内半乳糖积累，减少活性氧(ROS)生成，提示其在缓解半乳糖代谢异常相关疾病中的潜在应用价值。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“recombinant human GALK2”“galactokinase 2 expression”等获取近期研究。
Recombinant human galactokinase 2 (GALK2) is a key enzyme involved in the metabolism of galactose, a monosaccharide essential for energy production and cellular processes. GALK2 belongs to the galactokinase family and catalyzes the ATP-dependent phosphorylation of galactose to galactose-1-phosphate, a critical step in the Leloir pathway. Unlike its isoform GALK1. which is primarily expressed in the liver and associated with galactosemia (a rare genetic disorder), GALK2 exhibits broader tissue distribution, including the kidneys, brain, and heart, suggesting distinct physiological roles beyond classical galactose metabolism.
The recombinant form of GALK2 is produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling high-purity protein yields for research. Structural studies of recombinant GALK2 have revealed insights into its substrate-binding specificity and regulatory mechanisms, highlighting conserved motifs critical for catalytic activity. Researchers utilize this protein to investigate its potential involvement in metabolic disorders, including diabetes and age-related cataracts, as aberrant galactose metabolism may contribute to disease progression.
Additionally, recombinant GALK2 serves as a tool for drug discovery, particularly in designing inhibitors to modulate galactose-related pathways. Its role in cellular signaling and oxidative stress responses further underscores its relevance in biomedical research. By elucidating GALK2’s molecular functions, scientists aim to develop targeted therapies for metabolic diseases and improve diagnostic strategies for galactosemia variants.
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