| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAK |
| Uniprot No | O14976 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-416aa |
| 氨基酸序列 | DQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF |
| 分子量 | 68.97 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GAK蛋白的3篇具有代表性的文献示例及其摘要内容：
1. **文献名称**: "Expression and Purification of Recombinant Human GAK Protein for Kinase Activity Analysis"
**作者**: Zhang et al.
**摘要**: 该研究描述了利用大肠杆菌表达系统高效表达重组人GAK蛋白的方法，并通过亲和层析纯化获得高纯度蛋白。作者验证了其激酶活性，表明GAK对特定底物磷酸化具有依赖ATP的特性，为后续功能研究提供了工具。
2. **文献名称**: "Structural Insights into Cyclin G-Associated Kinase (GAK) by Cryo-EM"
**作者**: Lee et al.
**摘要**: 本研究通过冷冻电镜技术解析了重组人GAK蛋白的晶体结构，揭示了其激酶结构域与调控区域的构象变化机制。结构分析表明，GAK的ATP结合口袋具有潜在药物靶向性，为设计小分子抑制剂奠定了基础。
3. **文献名称**: "GAK in Clathrin-Mediated Endocytosis: Functional Studies Using Recombinant Protein Knockdown Models"
**作者**: Wang et al.
**摘要**: 通过体外重组人GAK蛋白与细胞模型结合实验，发现GAK在网格蛋白介导的内吞过程中起关键作用。研究利用siRNA敲低GAK后观察到内吞效率下降，而补充重组GAK可部分恢复功能，证实其在内吞调控中的必要性。
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**说明**：以上文献为示例性质，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文献，可进一步提供研究领域方向(如疾病机制、药物筛选等)。
**Background of Recombinant Human GAK Protein**
GAK (Cyclin G-Associated Kinase), also known as auxilin 2. is a ubiquitously expressed serine/threonine kinase involved in diverse cellular processes, including clathrin-mediated endocytosis, cell cycle regulation, and stress response. It interacts with cyclin G and Hsc70. playing a critical role in uncoating clathrin-coated vesicles during membrane trafficking. GAK also regulates the mitotic progression by modulating Aurora kinase B activity and spindle assembly.
Dysregulation of GAK is linked to neurodegenerative diseases (e.g., Parkinson’s), cancer, and viral infections. Its association with α-synuclein aggregation in Parkinson’s and promotion of cancer cell proliferation via kinase-dependent pathways highlight its therapeutic potential. Additionally, GAK facilitates viral entry (e.g., hepatitis C virus) by aiding clathrin-dependent endocytosis.
Recombinant human GAK protein, produced using prokaryotic or eukaryotic expression systems, retains functional domains—a kinase domain and an auxilin-like J-domain—enabling biochemical and structural studies. It serves as a tool to investigate GAK’s molecular mechanisms, screen inhibitors, and explore disease-related pathways. The recombinant form bypasses challenges of isolating native GAK, ensuring high purity and reproducibility for research and drug development applications.
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