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Recombinant Human FUNDC1 Protein

  • 中文名: 重组人FUNDC1蛋白
  • 别    名: FUNDC1; FUN14 domain-containing protein 1
货号: PA2000-7854
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点FUNDC1
Uniprot NoQ8IVP5
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-155aa
氨基酸序列MATRNPPPQDYESDDDSYEVLDLTEYARRHQWWNRVFGHSSGPMVEKYSVATQIVMGGVTGWCAGFLFQKVGKLAATAVGGGFLLLQIASHSGYVQIDWKRVEKDVNKAKRQIKKRANKAAPEINNLIEEATEFIKQNIVISSGFVGGFLLGLAS
分子量43.6 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于重组人FUNDC1蛋白的模拟参考文献示例(仅供参考,非真实文献):

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1. **"Structural basis of FUNDC1-LC3 interaction in mitophagy"**

*作者:Chen L., Zhang Q., et al. (2020)*

**摘要**:本研究通过重组表达人FUNDC1蛋白,结合X射线晶体学解析其结构,揭示了其LC3结合域的构象变化,阐明了FUNDC1在缺氧诱导的线粒体自噬中招募LC3的分子机制。

2. **"FUNDC1 regulates mitochondrial quality control in cardiomyocytes via ULK1-dependent pathway"**

*作者:Liu Y., Wang H., et al. (2019)*

**摘要**:通过体外重组FUNDC1蛋白实验,证明其与ULK1激酶的相互作用,激活线粒体自噬通路,保护心肌细胞免受缺血再灌注损伤,为心血管疾病治疗提供新靶点。

3. **"Recombinant FUNDC1 suppresses tumor growth by enhancing mitophagy-mediated apoptosis"**

*作者:Zhang R., Li X., et al. (2021)*

**摘要**:利用哺乳动物细胞系统表达重组人FUNDC1蛋白,证明其过表达通过促进线粒体自噬诱导肿瘤细胞凋亡,抑制体内外肿瘤生长,提示其潜在抗肿瘤应用价值。

4. **"Phosphorylation-dependent regulation of FUNDC1 function in mitochondrial dynamics"**

*作者:Wang S., Chen G., et al. (2022)*

**摘要**:研究重组人FUNDC1蛋白的磷酸化修饰(如Ser13位点)对其与Drp1蛋白结合的影响,揭示其在调控线粒体分裂和融合平衡中的关键作用。

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**注**:以上文献为模拟示例,实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。如需具体文献检索协助,可提供更详细的研究方向。


背景信息

FUNDC1 (FUN14 domain-containing protein 1) is a mitochondrial outer membrane protein crucial for hypoxia-induced mitophagy, a selective autophagy process that removes damaged mitochondria. It contains a conserved N-terminal transmembrane domain and a C-terminal domain that interacts with LC3 (autophagy-related protein) under low oxygen conditions, promoting mitochondrial clearance. FUNDC1-mediated mitophagy regulates mitochondrial quality control, energy metabolism, and cell survival, with implications in cardiovascular diseases, cancer, and neurodegenerative disorders.

The recombinant human FUNDC1 protein, typically expressed in *E. coli* or mammalian systems, retains functional domains for structural and interaction studies. Its production enables in vitro analysis of binding partners, post-translational modifications (e.g., phosphorylation regulating mitophagy), and mutational effects. Researchers use it to explore FUNDC1's role in disease models or therapeutic strategies targeting mitochondrial dysfunction. Current studies focus on its interplay with hypoxia signaling, apoptosis, and metabolic stress responses. Its recombinant form has become a key tool in deciphering mitophagy mechanisms and developing potential diagnostic or therapeutic agents.


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