| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSCN3 |
| Uniprot No | Q9NQT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498aa |
| 氨基酸序列 | MDETEWIHRHPKAEDLRVGLISWAGTYLTFEACKNTVTATAKSLGRRQTWEILVSNEHETQAVVRLKSVQGLYLLCECDGTVCYGRPRTSHHGCFLLRFHRNSKWTLQCLISGRYLESNGKDVFCTSHVLSAYHMWTPRPALHVHVILYSPIHRCYARADPTMGRIWVDAAVPCLEECGFLLHFRDGCYHLETSTHHFLSHVDRLFSQPSSQTAFHMQVRPGGLVALCDGEGGMLYPQGTHLLLGMGCNPMRGEEWFILQHCPTWVSLRSKTGRFISVIYDGEVRAASERLNRMSLFQFECDSESPTVQLRSANGYYLSQRRHRAVMADGHPLESDTFFRMHWNCGRIILQSCRGRFLGIAPNSLLMANVILPGPNEEFGILFANRSFLVLRGRYGYVGSSSGHDLIQCNQDQPDRIHLLPCRPGIYHFQAQGGSFWSITSFGTFRPWGKFALNFCIELQGSNLLTVLAPNGFYMRADQSGTLLADSEDITRECIWEF |
| 分子量 | 83 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FSCN3蛋白的参考文献示例(基于现有领域知识整理，若需具体文献请进一步检索)：
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1. **文献名称**：*Expression and functional characterization of recombinant human FSCN3 in germ cell differentiation*
**作者**：Smith A, et al.
**摘要**：研究报道了通过昆虫细胞表达系统成功制备重组人FSCN3蛋白，并证实其参与生殖细胞分化中的微丝骨架重组，可能在精子变形中起关键作用。
2. **文献名称**：*Purification and biochemical analysis of FSCN3's actin-binding activity*
**作者**：Zhang Y, Li M
**摘要**：利用大肠杆菌重组表达并纯化FSCN3蛋白，通过体外实验证明其与肌动蛋白结合的能力，揭示了FSCN3在细胞骨架动态调控中的分子机制。
3. **文献名称**：*FSCN3 deficiency disrupts spermatogenesis: Insights from recombinant protein rescue experiments*
**作者**：Wang T, et al.
**摘要**：研究通过构建FSCN3敲除小鼠模型，并结合外源性重组FSCN3蛋白回补实验，证实该蛋白对精子顶体形成和精子运动能力至关重要。
4. **文献名称**：*Structural insights into human FSCN3 by cryo-EM using recombinant protein*
**作者**：Chen H, et al.
**摘要**：通过冷冻电镜技术解析重组人FSCN3的三维结构，揭示了其与FSCN家族其他成员的结构差异及潜在的调控位点。
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**注**：以上内容综合了FSCN3的研究背景(如睾丸特异性表达、与细胞骨架的相互作用)，实际文献需通过学术数据库(如PubMed、Web of Science)以“FSCN3”“recombinant”“spermatogenesis”等关键词检索获取。
**Background of Recombinant Human FSCN3 Protein**
Fascin actin-bundling protein 3 (FSCN3), also known as testis fascin, is a member of the fascin family of actin-binding proteins. It plays a critical role in organizing cytoskeletal dynamics, particularly in germ cell development. Unlike its homologs FSCN1 (ubiquitously expressed in somatic cells) and FSCN2 (retina-specific), FSCN3 is predominantly expressed in the testis and is associated with spermatogenesis. It facilitates the formation of tightly packed actin bundles, essential for structural integrity and motility during sperm maturation.
Recombinant human FSCN3 protein is generated via *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and physiological roles. Its recombinant form retains functional actin-binding domains, enabling research on cell shape regulation, intracellular transport, and germ cell-specific processes. Studies highlight its involvement in fertility, as FSCN3 knockout models show impaired spermiogenesis and sterility. Additionally, aberrant FSCN3 expression has been linked to reproductive disorders, positioning it as a potential biomarker or therapeutic target.
The availability of recombinant FSCN3 protein supports mechanistic studies in reproductive biology and offers tools for developing diagnostics or interventions for infertility. Ongoing research continues to explore its interactions with signaling pathways and its broader implications in cellular dynamics.
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