| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSCB |
| Uniprot No | Q5H9T9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-825aa |
| 氨基酸序列 | MVGKSQQTDVIEKKKHMAIPKSSSPKATHRIGNTSGSKGSYSAKAYESIRVSSELQQTWTKRKHGQEMTSKSLQTDTIVEEKKEVKLVEETVVPEEKSADVREAAIELPESVQDVEIPPNIPSVQLKMDRSQQTSRTGYWTMMNIPPVEKVDKEQQTYFSESEIVVISRPDSSSTKSKEDALKHKSSGKIFASEQPEFQPATNSNEEIGQKNISRTSFTQETKKGPPVLLEDELREEVTVPVVQEGSAVKKVASAEIEPPSTEKFPAKIQPPLVEEATAKAEPRPAEETHVQVQPSTEETPDAEAATAVAENSVKVQPPPAEEAPLVEFPAEIQPPSAEESPSVELLAEILPPSAEESLSEEPPAEILPPPAEKSPSVEPLGEIRSPSAQKAPIEVQPLPAEGALEEASAKVEPPTVEETLADVQPLLPEEAPREEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADETPAEARSPLSEETSAEEAHAEVQSPLAEETTAEEASAEIQLLAAIEAPADETPAEAQSPLSEETSAEEAPAEVQSPSAKGVSIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEETPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQSLPAEETPIEETLAAVHSPPADDVPAEEASVDKHSPPADLLLTEEFPIGEASAEVSPPPSEQTPEDEALVENVSTEFQSPQVAGIPAVKLGSVVLEGEAKFEEVSKINSVLKDLSNTNDGQAPTLEIESVFHIELKQRPPEL |
| 分子量 | 114.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人FSCB(Fibrous Sheath CABYR-binding Protein)蛋白的相关文献信息概括：
1. **文献名称**：Expression and functional characterization of recombinant human FSCB in sperm motility regulation
**作者**：Zhang Y. et al.
**摘要**：该研究成功在大肠杆菌系统中表达重组人FSCB蛋白，并通过免疫荧光定位发现其在精子纤维鞘中富集。体外实验表明，FSCB通过结合钙信号相关蛋白调控精子尾部超活化运动。
2. **文献名称**：Structural insights into FSCB interaction with CABYR during fibrous sheath assembly
**作者**：Chen L. et al.
**摘要**：利用重组表达的FSCB和CABYR蛋白进行共结晶分析，揭示了二者结合的关键结构域，阐明了FSCB在精子纤维鞘组装中的支架作用，为男性不育机制提供了分子依据。
3. **文献名称**：FSCB phosphorylation dynamics during sperm capacitation: A proteomic approach
**作者**：Wang R. et al.
**摘要**：通过磷酸化蛋白质组学分析，发现重组人FSCB在精子获能过程中发生特异性磷酸化修饰，证实其磷酸化状态与精子活力及穿透卵子能力正相关。
注：实际文献需通过PubMed/Google Scholar等平台检索确认，以上为根据该领域研究的模拟概括。
Recombinant human FSCB (Fibrous Sheath CABYR-binding) protein is a genetically engineered protein derived from the human FSCB gene, which is predominantly expressed in the testis and plays a critical role in sperm development and function. FSCB is a structural component of the fibrous sheath, a cytoskeletal structure surrounding the axoneme in the sperm tail, essential for sperm motility and maturation. It interacts with CABYR (Calcium-Binding and Tyrosine Phosphorylation-Regulated Protein), contributing to the fibrous sheath's assembly and stabilization during spermiogenesis.
The recombinant FSCB protein is typically produced using bacterial or mammalian expression systems, enabling researchers to study its biochemical properties, molecular interactions, and role in sperm physiology. Its production facilitates investigations into male infertility, as mutations or dysregulation of FSCB have been linked to sperm structural abnormalities and reduced fertility. Additionally, recombinant FSCB serves as a potential antigen for developing contraceptive vaccines or diagnostic biomarkers for infertility screening.
Current research focuses on elucidating FSCB's phosphorylation-regulated interactions, calcium-binding domains, and its involvement in signaling pathways during sperm capacitation. These studies aim to advance reproductive medicine by identifying therapeutic targets for infertility or non-hormonal contraceptives.
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