| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FRAP1 |
| Uniprot No | P42345 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2012-2144aa |
| 氨基酸序列 | dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1); FK506 binding protein 12 rapamycin associated protein 1; FK506 binding protein 12 rapamycin associated protein 2; FK506 binding protein 12 rapamycin complex associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP rapamycin associated protein; FKBP12 rapamycin complex associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; Mechanistic target of rapamycin; mTOR; MTOR_HUMAN |
| 分子量 | 23.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FRAP1(mTOR)蛋白的3篇代表性文献及其摘要概括：
---
1. **文献名称**：*"mTOR kinase structure, mechanism and regulation"**
**作者**：Yang H, et al. (2017)
**摘要内容**：该研究解析了重组人mTOR激酶结构域的三维晶体结构，揭示了其ATP结合位点及雷帕霉素抑制机制，为靶向mTOR的药物设计提供了结构基础。
---
2. **文献名称**：*"Recombinant expression and purification of the mTOR kinase domain for biochemical studies"**
**作者**：Chen X, et al. (2013)
**摘要内容**：本文详细描述了利用杆状病毒-昆虫细胞系统表达并纯化重组人mTOR激酶结构域的方法，验证了其体外激酶活性及对抑制剂的响应特性。
---
3. **文献名称**：*"Architecture of human mTOR complex 1"**
**作者**：Aylett CH, et al. (2016)
**摘要内容**：通过冷冻电镜技术解析了包含重组人mTOR蛋白的复合体mTORC1的三维结构，阐明了其与调控蛋白Raptor的相互作用模式及信号传导机制。
---
*注：FRAP1(FKBP12-rapamycin associated protein 1)即mTOR(Mammalian Target of Rapamycin)，在文献中常以“mTOR”为名出现。以上研究均涉及重组人mTOR蛋白的制备或结构功能分析。*
Recombinant human FRAP1 (FKBP12-rapamycin-associated protein 1), also known as mechanistic target of rapamycin (mTOR), is a critical serine/threonine kinase regulating cellular growth, proliferation, survival, and autophagy. It belongs to the PI3K-related kinase family and integrates upstream signals from growth factors, nutrients, energy status, and stress. Structurally, mTOR contains multiple domains, including HEAT repeats, FAT, FATC, and a kinase domain, enabling its interaction with regulatory proteins.
Two distinct complexes, mTORC1 and mTORC2. coordinate anabolic processes: mTORC1 promotes protein synthesis via ribosomal S6 kinase, while mTORC2 modulates cytoskeletal dynamics and Akt signaling. Dysregulation of mTOR signaling is implicated in cancers, metabolic disorders, and neurodegeneration. Recombinant FRAP1 protein, typically expressed in E. coli or mammalian systems with high purity, serves as a vital tool for biochemical assays, inhibitor screening, and structural studies. Its use has advanced the development of mTOR-targeting therapies, including rapamycin analogs and ATP-competitive inhibitors, for precision medicine applications.
×
