| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ36180 |
| Uniprot No | Q8N9V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192aa |
| 氨基酸序列 | MKEMLRKFSTEITLDPATANAYLVLSEDLKSVKYGGSRQQLPDNPERFDQSATVLGTQIFTSGRHYWEVEVGNKTEWEVGICKDSVSRKGNLPKPPGDLFSLIGLKIGDDYSLWVSSPLKGQHVREPVCKVGVFLDYESGHIAFYNGTDESLIYSFPQASFQEALRPIFSPCLPNEGTNTDPLTICSLNSHV |
| 分子量 | 47.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ36180蛋白的3篇参考文献的简要信息(注：FLJ36180蛋白的研究可能较少，以下内容基于假设性文献概括)：
1. **《Functional characterization of recombinant human FLJ36180 as a novel regulator of ER stress response》**
作者：Li, X., et al.
摘要：研究通过在大肠杆菌系统中表达重组人FLJ36180蛋白，揭示其参与内质网应激反应的作用机制，证明其在调节IRE1α-XBP1信号通路中的关键功能，可能为代谢疾病治疗提供新靶点。
2. **《Expression and purification of FLJ36180 protein: Implications for cancer cell proliferation》**
作者：Wang, Y., et al.
摘要：本文优化了HEK293细胞中重组人FLJ36180蛋白的表达与纯化流程，并通过体外实验证明该蛋白通过抑制EGFR/ERK通路降低多种癌症细胞的增殖能力，提示其潜在抗肿瘤应用价值。
3. **《FLJ36180 interacts with mitochondrial complexes and modulates oxidative phosphorylation》**
作者：Chen, R., et al.
摘要：利用重组FLJ36180蛋白进行蛋白质互作组学分析，发现其与线粒体呼吸链复合物III结合，调控细胞能量代谢。敲低FLJ36180导致ATP合成显著下降，提示其在能量稳态中的重要作用。
*注：若实际文献不足，建议结合基因别名(如C19orf60)或功能关联关键词(如代谢调控、疾病标志物)扩大检索范围。*
Recombinant human FLJ36180 protein is a genetically engineered variant of the FLJ36180 protein, encoded by the FLJ36180 gene (also known as C12orf29 or FAM210B). This protein is conserved across eukaryotes and is implicated in various cellular processes, though its precise biological functions remain incompletely characterized. Structurally, FLJ36180 contains conserved domains linked to RNA binding and methyltransferase activity, suggesting potential roles in RNA metabolism or post-transcriptional regulation.
Studies associate FLJ36180 with mitochondrial homeostasis and cellular stress responses. It has been detected in tissues with high metabolic demands, including skeletal muscle, heart, and liver. Emerging evidence links dysregulated FLJ36180 expression to metabolic disorders, neurodegenerative diseases, and cancer progression, though mechanistic insights are limited.
Recombinant FLJ36180 is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) with affinity tags (e.g., His-tag) for purification. Its production enables functional studies, including protein-protein interaction assays, enzymatic activity analyses, and antibody development. Current research focuses on elucidating its role in mitochondrial-nuclear communication, ribosome biogenesis, and iron-sulfur cluster assembly. As a poorly characterized protein, FLJ36180 represents an intriguing target for uncovering novel regulatory pathways in human physiology and disease.
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