| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ35220 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MALEAAGGPPEETLSLWKREQARLKAHVVDRDTEAWQRDPAFSGLQRVGGVDVSFVKGDSVRACASLVVLSFPELEVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHKEKIRLLQTRGDSFPLLGDSGTVLGMALRSHDRSTRPLYISVGHRMSLEAAVRLTCCCCRFRIPEPVRQADICSREHIRKSLGLPGPPTPRSPKAQRPVACPKGDSGESSGEGQPPQDHSPGPRTAPRPGSQEQAGKDWQ |
| 分子量 | 54.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ35220蛋白的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索验证)：
1. **文献名称**: *Expression and Purification of Recombinant Human FLJ35220 in E. coli*
**作者**: Zhang L. et al.
**摘要**: 本研究成功构建了FLJ35220蛋白的大肠杆菌表达系统，通过His标签亲和层析纯化，获得高纯度重组蛋白，并通过质谱鉴定确认其正确性，为后续功能研究奠定基础。
2. **文献名称**: *Functional Analysis of FLJ35220 in Apoptosis Regulation*
**作者**: Kim S., Park H.
**摘要**: 发现重组人FLJ35220蛋白通过调控Bcl-2家族蛋白表达抑制癌细胞凋亡，体外实验表明其过表达可增强HeLa细胞对化疗药物的抵抗性。
3. **文献名称**: *Crystal Structure of FLJ35220 Reveals a Novel α-Helical Domain*
**作者**: Müller F. et al.
**摘要**: 首次解析了FLJ35220蛋白的晶体结构(分辨率2.8Å)，发现其具有独特的α螺旋结构域，暗示其在蛋白-蛋白相互作用中的潜在作用。
4. **文献名称**: *FLJ35220 as a Biomarker in Chronic Inflammation*
**作者**: Chen R. et al.
**摘要**: 通过ELISA检测显示，重组FLJ35220在类风湿关节炎患者血清中显著高表达，提示其可能作为炎症性疾病的诊断标志物。
建议通过 **PubMed** 或 **Web of Science** 以实际检索词(如“FLJ35220 recombinant”、“C14orf105 protein”)获取真实文献。
Recombinant human FLJ35220 protein is a genetically engineered protein derived from the FLJ35220 gene, which encodes a hypothetical protein with limited functional characterization. The FLJ35220 gene, also designated as C3orf70. is located on chromosome 3 and is conserved across vertebrates, suggesting potential biological importance. Its transcript encodes a polypeptide of approximately 30 kDa, predicted to contain disordered regions and possible functional motifs, though detailed structural analysis remains sparse. Current bioinformatics studies associate FLJ35220 with cellular metabolism and signal transduction pathways, particularly in energy homeostasis, but experimental validation is lacking. Recombinant FLJ35220 is typically expressed in prokaryotic systems (e.g., E. coli) with affinity tags to enable purification, facilitating studies on its biochemical properties and interactome. Researchers have explored its potential roles in mitochondrial function and cell cycle regulation through overexpression/knockdown models, though mechanistic insights are preliminary. The protein's involvement in disease contexts, such as cancer or metabolic disorders, remains speculative but is under investigation. Commercial availability of recombinant FLJ35220 supports antibody development and functional assays. Challenges include clarifying post-translational modifications in native tissues and establishing standardized detection methods. This protein represents an emerging target for decoding uncharacterized human proteome elements.
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