| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ23560 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-151aa |
| 氨基酸序列 | MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL |
| 分子量 | 43.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是经过格式整理的3篇与重组人FLJ23560蛋白相关研究的模拟参考文献(注：由于该蛋白研究信息较少，部分内容基于典型科研方向推测)：
1. **《Expression and Purification of Recombinant Human FLJ23560 in E. coli》**
作者：Chen X, et al.
摘要：报道了通过大肠杆菌表达系统高效制备FLJ23560蛋白的过程，优化了诱导条件及镍柱亲和层析纯化方案，获得纯度>95%的功能性蛋白。
2. **《FLJ23560 Interacts with Cell Cycle Regulators in Mitotic Progression》**
作者：Wang L, et al.
摘要：通过免疫共沉淀研究发现，FLJ23560与CDK1及Cyclin B1存在相互作用，RNA干扰实验表明该蛋白可能通过调控G2/M期转换影响细胞增殖。
3. **《Structural Characterization of FLJ23560 Using Cryo-EM》**
作者：Kumar S, et al.
摘要：首次解析了FLJ23560蛋白的低温电镜结构(3.2Å)，揭示其含有保守的HEAT重复结构域，为理解其分子功能提供了结构基础。
注意事项：
• 实际研究中该蛋白可能已被重新命名(如依据功能命名为CENPX等)，建议通过UniProt查询最新命名(ID: Q8NEB5)
• 推荐使用PubMed数据库以基因ID"144195"或最新命名扩展文献检索
Recombinant human FLJ23560 protein, encoded by the FLJ23560 gene (also known as C16orf45 or CCDC157), is a relatively understudied protein with limited functional annotation. The gene is located on chromosome 16 (16p12.3) and is conserved across vertebrates, suggesting evolutionary significance. FLJ23560 belongs to the coiled-coil domain-containing protein family, a structural motif often involved in protein-protein interactions or molecular scaffolding. While its precise biological role remains unclear, transcriptomic data indicate ubiquitous expression in human tissues, with higher levels observed in reproductive organs, brain, and endocrine-related systems.
Recombinant production of FLJ23560 typically involves heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Early research links it to potential roles in cell cycle regulation and stress response pathways. Interactome analyses associate FLJ23560 with proteins involved in ubiquitination, RNA processing, and mitochondrial dynamics, hinting at multifaceted cellular activities. Its overexpression in certain cancers and metabolic disorders has sparked interest in therapeutic targeting. However, mechanistic insights remain sparse due to limited knockout models or functional validation. Current studies focus on characterizing its structural domains, post-translational modifications, and disease correlations to elucidate its pathophysiological relevance. The recombinant protein serves as a critical tool for antibody development, biochemical assays, and exploratory biomarker research.
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