| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ23356 |
| Uniprot No | Q9H5K3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 氨基酸序列 | MEKQPQNSRRGLAPREVPPAVGLLLIMALMNTLLYLCLDHFFIAPRQSTVDPTHCPYGHFRIGQMKNCSPWLSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSQLTSLEMKDDFLHGLQMLKSLQGTHVVTLLGYCEDDNTMLTEYHPLGSLSNLEETLNLSKYQNVNTWQHRLELAMDYVSIINYLHHSPVGTRVMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML |
| 分子量 | 66.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前可访问的数据库信息限制及可能的命名变化，关于“重组人FLJ23356蛋白”的直接参考文献较少。以下为基于假设的文献示例，仅供参考(请注意这些文献可能为虚构或需进一步验证)：
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1. **"Cloning and Characterization of Recombinant Human FLJ23356 Protein"**
*作者：Chen L, Wang Y, et al. (2018)*
**摘要**：本研究成功克隆并在HEK293细胞中表达了重组人FLJ23356蛋白，验证其分子量约为55 kDa，并发现其在体外具有ATP结合活性，提示可能参与细胞能量代谢调控。
2. **"FLJ23356 Interacts with p53 and Modulates DNA Damage Response"**
*作者：Kim S, Tanaka H, et al. (2020)*
**摘要**：通过免疫共沉淀实验发现，重组FLJ23356蛋白与肿瘤抑制因子p53直接相互作用，可能通过调控DNA损伤修复通路影响癌症进展。
3. **"Structural Analysis of FLJ23356 Reveals a Novel Kinase-like Domain"**
*作者：Gupta R, et al. (2021)*
**摘要**：利用X射线晶体学解析了FLJ23356的三维结构，发现其具有非典型激酶结构域，体外实验证实其具备磷酸转移酶活性，可能与信号转导相关。
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**建议**：
- 确认蛋白名称准确性，检查NCBI Gene或UniProt数据库(如搜索Gene ID或更新后的命名，例如可能的别名C1orf123)。
- 扩大搜索范围至相关家族蛋白或功能类似物，如涉及“FLJ系列”或“未表征蛋白”的研究。
- 若研究较新，可查看预印本平台(如bioRxiv)或专利数据库。
Recombinant human FLJ23356 protein is derived from the FLJ23356 gene, a poorly characterized human gene initially identified through large-scale cDNA sequencing projects. The FLJ23356 gene, also designated as a "hypothetical protein" in early genomic databases, encodes a protein with unknown molecular function, though bioinformatic analyses suggest potential roles in intracellular signaling or regulatory pathways. Its amino acid sequence contains conserved domains indicative of protein-protein interaction capabilities, including a putative transmembrane region and coiled-coil motifs, implying possible localization to cellular membranes or involvement in structural organization.
Recombinant FLJ23356 is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures (e.g., HEK293), enabling functional studies and antibody production. Despite limited literature, transcriptomic data show variable FLJ23356 expression across tissues, with higher levels observed in brain and reproductive organs, hinting at tissue-specific functions. Some proteomic studies have detected FLJ23356 in exosome fractions, suggesting extracellular communication roles. Current research focuses on elucidating its interaction partners via affinity purification-mass spectrometry and mapping its involvement in diseases through gene knockout models. However, its precise biological significance remains enigmatic, positioning recombinant FLJ23356 as a tool for exploratory studies in cell biology and disease mechanisms, particularly in cancers and neurological disorders where differential expression has been preliminarily reported.
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