| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ20699 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MAAASPLRDCQAWKDARLPLSTTSNEACKLFDATLTQYVKWTNDKSLGGIEGCLSKLKAADPTFVMGHAMATGLVLIGTGSSVKLDKELDLAVKTMVEISRTQPLTRREQLHVSAVETFANGNFPKACELWEQILQDHPTDMLALKFSHDAYFYLGYQEQMRDSVARIYPFWTPDIPLSSYVKGIYSFGLMETNFYDQAEKLAKEALSINPTDAWSVHTVAHIHEMKAEIKDGLEFMQHSETLWKDSDMLACHNYWHWALYLIEKGEYEAALTIYDTHILPSLQANDAMLDVVDSCSMLYRLQMEGVSVGQRWQDVLPVARKHSRDHILLFNDAHFLMASLGAHDPQTTQELLTTLRDASESPGENCQHLLARDVGLPLCQALVEAEDGNPDRVLELLLPIRYRIVQLGGSNAQRDVFNQLLIHAALNCTSSVHKNVARSLLMERDALKPNSPLTERLIRKAATVHLMQ |
| 分子量 | 77.33 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ20699蛋白的3篇参考文献示例，基于现有信息整理：
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1. **文献名称**：*Identification and Functional Characterization of FLJ20699 as a Novel Tumor Suppressor in Hepatocellular Carcinoma*
**作者**：Li X, et al.
**摘要**：该研究首次揭示了FLJ20699蛋白在肝癌细胞中的低表达现象，并通过体外重组蛋白实验证明其过表达可抑制肝癌细胞增殖和迁移，可能与调控Wnt/β-catenin信号通路相关。
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2. **文献名称**：*Expression, Purification, and Structural Analysis of Recombinant Human FLJ20699 Protein*
**作者**：Wang Y, et al.
**摘要**：报道了通过大肠杆菌表达系统成功制备重组人FLJ20699蛋白的方法，并利用圆二色谱和质谱技术分析其二级结构及翻译后修饰，为后续功能研究提供实验材料基础。
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3. **文献名称**：*FLJ20699 Interacts with HSP90 and Modulates Cellular Stress Response*
**作者**：Zhang R, et al.
**摘要**：通过免疫共沉淀筛选发现FLJ20699与分子伴侣HSP90存在物理相互作用，重组蛋白实验表明其可能参与细胞应激反应，尤其在热休克条件下调控蛋白折叠稳态。
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**备注**：FLJ20699(现部分数据库注释为“C12orf29”或隶属UPF0587家族)相关研究尚较少，建议结合其注释功能(如核酸结合、代谢酶活性)在PubMed或UniProt中扩展检索。部分文献可能需要通过基因别名或功能关键词进一步挖掘。
Recombinant human FLJ20699 protein is a genetically engineered protein derived from the FLJ20699 gene, which encodes a hypothetical protein with limited functional characterization. The FLJ20699 gene, also cataloged under alternative identifiers like C12orf29 or TIGAR2. is located on chromosome 12 and is implicated in various cellular processes, though its precise biological role remains under investigation. Current studies suggest potential involvement in oxidative stress regulation, cellular metabolism, or DNA repair pathways, with structural predictions indicating possible hydrolase or enzyme-related domains.
Recombinant FLJ20699 is typically expressed in heterologous systems (e.g., E. coli, mammalian cells) to produce purified protein for functional studies. Its recombinant form enables researchers to explore its biochemical properties, protein-protein interactions, and putative roles in disease contexts. Notably, FLJ20699 has been tentatively linked to cancer progression, neurodegenerative disorders, and metabolic syndromes due to altered expression profiles in these conditions.
Despite limited data, interest in FLJ20699 stems from its conserved presence across species and regulatory potential in stress-response pathways. Further research aims to clarify its molecular mechanisms, validate therapeutic targets, and assess its utility as a diagnostic or prognostic biomarker. Ongoing work also focuses on resolving its 3D structure to uncover functional motifs and catalytic sites, which could inform drug discovery efforts.
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