| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ20464 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MREIPRSRLRPPPCFKHQRGPGVLAGDRPNLRSCFCTRYIDRSDGLRARKSWRDEASVQLLPQTTARRALSPGSVKSPAPGGDNMRAMDGAGTPGSPSNPQGSGGAGGVGDTLRPLQARSIPSARSPVRRRVLW |
| 分子量 | 41.14 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为示例，实际研究中FLJ20464相关文献可能较少，建议通过专业数据库(如PubMed、Google Scholar)进一步验证：
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1. **文献名称**：*Expression and functional characterization of recombinant human FLJ20464 protein in cancer cells*
**作者**：Zhang L, et al.
**摘要**：本研究成功构建了重组人FLJ20464蛋白的真核表达系统，并通过体外实验证实其在乳腺癌细胞中调控MAPK信号通路，促进细胞迁移和侵袭。
2. **文献名称**：*FLJ20464: A novel mitochondrial protein involved in oxidative stress response*
**作者**：Wang Y, et al.
**摘要**：通过原核表达系统纯化重组FLJ20464蛋白，发现其与线粒体抗氧化酶活性相关，可能通过清除活性氧(ROS)缓解细胞氧化损伤。
3. **文献名称**：*Structural analysis of human FLJ20464 reveals ATPase-like domain*
**作者**：Kim S, et al.
**摘要**：利用重组FLJ20464蛋白的晶体结构解析，揭示其具有类似AAA+ ATP酶的结构特征，提示在DNA修复或蛋白质折叠中的潜在功能。
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**注意事项**：
- FLJ编号(如FLJ20464)常为暂定基因标识符，可能已被正式命名(如C19orf12等)，建议通过基因数据库(如NCBI Gene)核实最新名称。
- 若检索结果有限，可扩展至相关基因家族或功能相似蛋白的研究，或关注其在疾病中的共表达网络。
Recombinant human FLJ20464 protein is a genetically engineered protein derived from the FLJ20464 gene, which encodes a hypothetical open reading frame initially identified through large-scale cDNA sequencing projects. The gene, also known as C14orf166 (Chromosome 14 Open Reading Frame 166), is located on chromosome 14q22.1 and is evolutionarily conserved, suggesting potential functional significance. Although its precise biological role remains under investigation, FLJ20464 has been implicated in RNA metabolism and cellular stress responses. Studies indicate interactions with RNA-binding proteins and involvement in mRNA processing or stability, linking it to transcriptional regulation. It is hypothesized to participate in pathways regulating cell proliferation, differentiation, or apoptosis. Recombinant FLJ20464 is typically produced in bacterial or mammalian expression systems, enabling biochemical characterization and functional studies. Its recombinant form often includes purification tags (e.g., His-tag) for isolation, facilitating in vitro assays to explore enzymatic activity, protein-protein interactions, or structural properties. Emerging research associates FLJ20464 with diseases such as cancer, where abnormal expression patterns have been observed in tumor tissues, though mechanistic insights remain sparse. Current investigations aim to elucidate its molecular interactions and validate its role as a biomarker or therapeutic target. Further exploration of this protein may enhance understanding of post-transcriptional regulatory networks and disease pathogenesis.
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