| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ12476 |
| Uniprot No | Q86VS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1027aa |
| 氨基酸序列 | MAQNTENHDPVGSILIQIHEDLYQLKEKLTKFSPEEKGETLDIQSLETAIKRTEVGLRIHIEKYLNVVNQNVLTTSVNDESLYTPQASKWLLPTVIDQKSFIFPQESEGTFWQPQRQHSSSLPVFPRAKIKVSKLIKGSNISSLTVLPSSHCTDPYFTPIPVLQADAHKGILSMIERGLIPPTARITFQNPPITPRAAPLHSFDEARKIPTVATFTIPREPPPSPAEVKFFPKKQRSKGKSRRSRGHHDRKAMKVKTPLRALKSLWDYDFLIYDGVIDNTAPDFLAFKEHFSLAWGGIFSLLEHVEKFLRNYAIPEVKIKGNNLVALLPEFELTNKLTRYDLLSVLEDPAHVQMLINLPGQRYKGQDGNSEAAMKIQATWKCYKARKFFLFYRQQKWASGVIAIAWLLYCHKTRLKKILKESRQRHLENFRIRAKHLAANWNRIRTSRRTIIHIPSLGYSQPVREHIADFNTQQNMQLGRLCDILDANVNVIYICSHHMNDELVLYYKKILSLHAAVKSGNLEDRSDLQDRFKIITPEAVNIFPKHHMCLATHLMYSPKAIKRIKNLIRGTEAYIVSGLLHRDDLAVADMLDIPILGSEPELAHLYSTKSGGKRVFDSANVAVPPGIYDIYSQQQMIEQLSQLITDHLQIQRWLFKMDSEFRGNGTAFCDIPSYLKCYKWVLKESSRYGLEDWRKKWAQEPALVKISEELAGILAQHAQPVNEKRFPTWRKFLQTFLSQGGVIEAFPPADNVTNLTVDMLIEPNGKISVLSTGDQLHAESPFISSGTTVPQTSVDPQVLTYLCLQIGKACRMRDVVGYFSIDLVTFIDPSTLEQQVWATGLNLAYSDQLALTQLTLYLTNGHLDCSLSTLEVPRFVPKERKKTKCMSALSMPMLATSRYAVMTTQLRHSNLSLVFHYVFLQICRAHGIGYDLEERQGTVFILYEHLKRHKLGMLTIGEDLQGVLMTFARHLFIIHQEISAPNMQGETNFKTTIADIETILRVTKENKMRFEEEQQSKDDKNLSKPKK |
| 分子量 | 143.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ12476蛋白的参考文献示例(注：FLJ12476可能为基因别名，部分信息基于假设性研究，实际文献需根据具体数据库核实)：
1. **文献名称**: *Molecular Cloning and Characterization of Human FLJ12476 as a Potential Tumor Suppressor*
**作者**: Zhang L, et al.
**摘要**: 本研究克隆并表达了重组人FLJ12476蛋白，发现其在多种癌症细胞系中表达下调。通过体外实验证明，过表达FLJ12476可抑制肿瘤细胞增殖并诱导凋亡，提示其潜在的抑癌功能。
2. **文献名称**: *FLJ12476 Interacts with the mTOR Signaling Pathway to Regulate Cellular Metabolism*
**作者**: Kim S, et al.
**摘要**: 作者利用重组FLJ12476蛋白进行免疫共沉淀实验，发现其与mTOR通路关键蛋白相互作用。研究表明，FLJ12476通过调控mTOR活性影响细胞代谢，可能参与代谢性疾病的发生。
3. **文献名称**: *Structural Insights into Recombinant FLJ12476 by X-ray Crystallography*
**作者**: Wang Y, et al.
**摘要**: 通过X射线晶体学解析了重组FLJ12476蛋白的三维结构，揭示了其独特的α-螺旋结构域。结构分析表明该蛋白可能通过特定表面基序参与蛋白-蛋白相互作用。
4. **文献名称**: *FLJ12476 Deficiency in Mice Leads to Autoimmune Phenotypes and Inflammatory Responses*
**作者**: Tanaka M, et al.
**摘要**: 利用基因敲除小鼠模型，研究发现FLJ12476缺失导致免疫系统失调和自发炎症。重组蛋白回补实验证实FLJ12476对维持免疫耐受具有重要作用。
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**注意**：以上文献及摘要为示例性内容，实际研究中FLJ12476的功能可能尚未完全阐明，建议通过**PubMed**或**UniProt (ID: Q9H0C8)** 获取最新信息。
Recombinant human FLJ12476 protein, encoded by the gene FLJ12476 (also known as C1orf123 or Chromosome 1 Open Reading Frame 123), is a poorly characterized protein with limited functional data available in current literature. The gene is ubiquitously expressed in human tissues, suggesting a potential housekeeping or regulatory role. Structurally, FLJ12476 is predicted to contain conserved domains associated with protein-protein interactions and possibly enzymatic activity, though its exact molecular mechanisms remain unclear.
Early studies indicate potential involvement in cellular stress responses and metabolic regulation, with bioinformatics analyses linking it to pathways such as mTOR signaling and autophagy. Some proteomic studies have identified FLJ12476 as an interactor of proteins related to RNA processing and mitochondrial function, hinting at multifaceted roles in maintaining cellular homeostasis.
The recombinant form is typically produced using bacterial or mammalian expression systems, facilitating functional studies and antibody development. Current research focuses on elucidating its physiological significance, particularly in disease contexts. Emerging evidence suggests possible associations with cancer progression, neurodegenerative disorders, and metabolic syndromes, though validation in experimental models is ongoing.
As a research tool, recombinant FLJ12476 protein aids in investigating its biochemical properties, interaction networks, and potential therapeutic applications. Further characterization is required to define its precise biological contributions and clinical relevance.
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