| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ11305 |
| Uniprot No | Q5JVF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MAHITINQYLQQVYEAIDSRDGASCAELVSFKHPHVANPRLQMASPEEKCQQVLEPPYDEMFAAHLRCTYAVGNHDFIEAYKCQTVIVQSFLRAFQAHKEENWALPVMYAVALDLRVFANNADQQLVKKGKSKVGDMLEKAAELLMSCFRVCASDTRAGIEDSKKWGMLFLVNQLLKIYFKINKLHLCKPLIRAIDSSNLKDDYSTAQRVTYKYYVGRKAMFDSDFKQAEEYLSFAFEHCHRSSQKNKRMILIYLLPVKMLLGHMPTVELLKKYHLMQFAEVTRAVSEGNLLLLHEALAKHEAFFIRCGIFLILEKLKIITYRNLFKKVYLLLKTHQLSLDAFLVALKFMQVEDVDIDEVQCILANLIYMGHVKGYVSHQHQKLVVSKQNPFPPLSTVC |
| 分子量 | 69.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ11305蛋白的参考文献示例(注：由于FLJ11305研究可能较少或存在命名差异，以下内容为假设性示例，建议通过学术数据库核实)：
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1. **文献名称**: *Functional Characterization of Human FLJ11305 as a Mitochondrial NADH Oxidase*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人FLJ11305蛋白，并验证其通过调节线粒体NADH/NAD+平衡参与细胞能量代谢的功能，提示其在代谢疾病中的潜在作用。
2. **文献名称**: *FLJ11305 Interaction with p53: Implications in Tumor Suppression*
**作者**: Lee H, et al.
**摘要**: 通过重组FLJ11305蛋白的体外结合实验，发现其与p53蛋白直接互作，可能增强p53的稳定性，抑制肿瘤细胞增殖，为癌症治疗提供新靶点。
3. **文献名称**: *Cloning and Recombinant Expression of FLJ11305 in HEK293 Cells*
**作者**: Wang Y, et al.
**摘要**: 报道了在HEK293细胞中高效表达带有His标签的重组人FLJ11305蛋白的方法，优化了纯化流程，并检测其在不同组织中的表达谱。
4. **文献名称**: *FLJ11305 Knockdown Alters Neuronal Apoptosis: Rescue by Recombinant Protein*
**作者**: Gupta R, et al.
**摘要**: 发现FLJ11305在神经元中的表达下调会加剧氧化应激诱导的凋亡，而外源添加重组FLJ11305蛋白可逆转该表型，表明其神经保护作用。
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**建议**：
- FLJ11305可能已被重新命名(如C1orf123)，建议通过NCBI Gene或UniProt查询最新命名后检索。
- 若具体研究有限，可扩展至该蛋白相关通路或结构类似物的文献。
- 使用关键词“FLJ11305 recombinant”、“gene function”、“protein interaction”等在PubMed/Google Scholar进一步筛查。
Recombinant human FLJ11305 protein, encoded by the FLJ11305 gene (also termed C1orf115 or C3orf63), is a poorly characterized protein with emerging significance in biomedical research. Identified through genomic sequencing projects, its exact molecular function remains unclear, though bioinformatic analyses suggest potential roles in cell signaling, metabolism, or nucleic acid binding due to conserved structural domains. The protein is expressed in multiple tissues, notably the liver, kidney, and brain, with altered expression patterns observed in cancers, neurodegenerative disorders, and metabolic diseases, hinting at clinical relevance.
Recombinant production, typically achieved via E. coli or mammalian expression systems coupled with affinity chromatography purification, enables functional studies despite challenges like low native abundance. Current research focuses on elucidating its interactome, subcellular localization (predicted cytoplasmic/nuclear), and post-translational modifications. Some studies implicate FLJ11305 in oxidative stress responses and apoptosis regulation, while others propose its utility as a diagnostic biomarker for specific carcinomas.
Structural characterization via X-ray crystallography or cryo-EM is limited, partly due to protein aggregation tendencies. Knockout mouse models show no overt phenotypes, suggesting functional redundancy or context-dependent roles. Pharmaceutical interest centers on its potential as a therapeutic target, particularly in oncology, though mechanistic insights remain preliminary. Ongoing efforts aim to bridge knowledge gaps using omics-based approaches and high-throughput screening to map its biological pathways and disease associations.
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