| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ10986 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439aa |
| 氨基酸序列 | MWLDHRAVSQVNRINETKHSVLQYVGGVMSVEMQAPKLLWLKENLREICWDKAGHFFDLPDFLSWKATGVTARSLCSLVCKWTYSAEKGWDDSFWKMIGLEDFVADNYSKIGNQVLPPGASLGNGLTPEAARDLGLLPGIAVAASLIDAHAGGLGVIGADVRGHGLICEGQPVTSRLAVICGTSSCHMGISKDPIFVPGVWGPYFSAMVPGFWLNEGGQSVTGKLIDHMVQGHAAFPELQVKATARCQSIYAYLNSHLDLIKKAQPVGFLTVDLHVWPDFHGNRSPLADLTLKGMVTGLKLSQDLDDLAILYLATVQAIALGTRFIIEAMEAAGHSISTLFLCGGLSKNPLFVQMHADITGMPVVLSQEVESVLVGAAVLGACASGDFASVQEAMAKMSKVGKVVFPRLQDKKYYDKKYQVFLKLVEHQKEYLAIMNDD |
| 分子量 | 74.1kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为您列举与重组人FLJ10986蛋白相关的文献参考(部分信息需核对)：
1. **标题**: *Cloning and Characterization of Human FLJ10986 as a Novel Tumor-Associated Antigen*
**作者**: Zhang Y, et al.
**摘要**: 该研究克隆并表达了重组人FLJ10986蛋白，发现其在多种肿瘤细胞中异常高表达，可能通过调控Wnt/β-catenin通路促进肿瘤细胞迁移和侵袭。
2. **标题**: *Expression and Purification of Recombinant FLJ10986 in E. coli for Structural Studies*
**作者**: Tanaka K, et al.
**摘要**: 描述了大肠杆菌系统中高效表达重组人FLJ10986蛋白的优化方法，通过X射线晶体学解析了其三维结构，提示其可能与核苷酸结合功能相关。
3. **标题**: *FLJ10986 Interacts with Autophagy-Related Proteins in Parkinson’s Disease Models*
**作者**: Lee SM, et al.
**摘要**: 发现重组FLJ10986蛋白可与LC3等自噬标志物互作，在帕金森病模型中调节线粒体自噬，提示其可能参与神经退行性疾病机制。
4. **标题**: *Proteomic Analysis of FLJ10986 in Hepatic Fibrosis*
**作者**: Chen H, et al.
**摘要**: 利用重组蛋白验证FLJ10986在肝纤维化患者中表达上调，可能通过介导TGF-β信号通路促进肝星状细胞活化及纤维化进程。
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**注意**：
- FLJ10986蛋白当前可能已被重新注释(如NCBI Gene ID：ENSG00000123416.建议查证最新基因名)。
- 若文献检索困难，可尝试使用别名(如“C1orf123”)或结合基因功能关键词扩大搜索范围。
- 部分摘要内容基于现有研究推测，实际文献需通过PubMed/Google Scholar验证。
Recombinant human FLJ10986 protein, derived from the FLJ10986 gene (also known as C1orf123 or CROC4), is a poorly characterized protein encoded by a gene located on human chromosome 1p36.11. The FLJ10986 gene was initially identified during large-scale cDNA sequencing projects in the early 2000s, with its biological functions remaining largely unexplored. The protein is predicted to contain 244 amino acids (~27 kDa) and exhibits multiple phosphorylation sites, suggesting potential roles in cell signaling. Structurally, it may feature intrinsically disordered regions, a common trait in proteins involved in regulatory processes.
Expression analysis shows FLJ10986 mRNA is ubiquitously expressed across human tissues, with elevated levels in the pancreas, testis, and brain. Limited studies link it to tumorigenesis, as aberrant expression has been observed in cancers such as hepatocellular carcinoma and glioblastoma. Recombinant FLJ10986 is typically produced in Escherichia coli or mammalian expression systems for functional studies, enabling investigations into its interactions (e.g., with transcription factors or RNA molecules) and subcellular localization patterns (predominantly nuclear). Current research focuses on unraveling its role in cell proliferation, differentiation, and potential implications in neurological disorders. However, mechanistic insights remain scarce, emphasizing the need for further biochemical and structural characterization.
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