关键信息
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基因名
UBA3
- 应用
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别名
UBA3；UBE1C；NEDD8-activating enzyme E1 catalytic subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TBC4
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表达区间
1-463aa
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氨基酸序列
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGP FTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVID MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQD FNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLID GGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCI EYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYR LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDG LYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSP AITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT TPQTVLFKLHFTS
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分子量
73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
UBA3 is a key enzyme involved in the ubiquitin-proteasome system, which plays a critical role in protein degradation and regulation within cells. As a member of the E1 enzyme family, UBA3 activates ubiquitin and transfers it to E2 conjugating enzymes, thus initiating the ubiquitination process that tags proteins for proteolytic degradation. Research into UBA3 has gained momentum due to its implications in various cellular processes, including cell cycle regulation, DNA repair, and apoptosis. Abnormalities in ubiquitination pathways have been linked to multiple diseases, including cancer, neurodegenerative disorders, and immune dysfunctions. Consequently, understanding the structure and function of the UBA3 protein could provide insights into its role in these conditions and contribute to the development of innovative therapeutic strategies. Recent studies have focused on characterizing UBA3's enzymatic activity, identifying its substrates, and elucidating its interaction with other components of the ubiquitin pathway. Investigating UBA3’s potential as a drug target is of particular interest, as modulation of its activity could offer novel approaches to treat diseases associated with proteostasis imbalance. Overall, the research surrounding UBA3 recombinant protein aims to unravel the complexities of ubiquitination and its broader implications in cellular health and disease.
