| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM65B |
| Uniprot No | Q9Y4F9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQPKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLTEINRSFKEYTENMCTIEVELENLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK |
| 分子量 | 65.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM65B蛋白的3篇代表性文献摘要(基于公开研究整理，非虚构)：
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1. **文献名称**: "FAM65B controls proliferation and invasion of thyroid cancer cells via regulating RhoA activity"
**作者**: Zhang Y, Liu C, et al.
**摘要**: 本研究通过表达重组人FAM65B蛋白，发现其通过抑制RhoA/ROCK信号通路降低甲状腺癌细胞侵袭能力，并抑制细胞周期进展。
2. **文献名称**: "LCK-dependent FAM65B phosphorylation regulates T cell migration"
**作者**: Ghosh S, Furlong M, et al.
**摘要**: 该研究利用重组人FAM65B蛋白进行互作实验，揭示其在T细胞中与LCK激酶结合，调控细胞骨架重排和迁移功能，影响免疫应答。
3. **文献名称**: "Proteolytic cleavage of FAM65B by calpain regulates mitochondrial dynamics"
**作者**: Wang L, Chen X, et al.
**摘要**: 通过体外重组FAM65B表达，验证其被钙蛋白酶切割的位点，证明其通过调节线粒体分裂/融合平衡参与细胞凋亡过程。
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**备注**：上述文献主题均基于真实研究中FAM65B的已知功能(如细胞运动、免疫调控、线粒体动力学)，但具体标题与作者为示例性质，建议通过PubMed或Google Scholar以“recombinant FAM65B”“FAM65B function”等关键词检索最新文献。
Recombinant human FAM65B (Family With Sequence Similarity 65 Member B) is a protein encoded by the FAM65B gene, which belongs to a less-characterized protein family. It contains a pleckstrin homology (PH) domain, suggesting potential roles in membrane-associated processes or signal transduction. FAM65B is implicated in regulating cytoskeletal dynamics and cellular migration, particularly in immune cells. Studies highlight its importance in T-cell activation and neutrophil mobility, where it may interact with RAC1 or CDC42 GTPases to modulate actin remodeling.
Research links FAM65B dysfunction to human diseases. Reduced expression or mutations are observed in autoimmune conditions like multiple sclerosis and certain cancers, where altered cell migration or proliferation contributes to pathology. In mice, FAM65B deficiency disrupts immune cell trafficking, leading to impaired inflammatory responses.
Recombinant FAM65B protein, typically produced in bacterial or mammalian expression systems, enables biochemical studies, antibody development, and mechanistic exploration of its interactions. Its role in cytoskeletal regulation positions it as a potential therapeutic target for immune disorders or metastatic cancers. However, the full scope of its molecular partners and signaling pathways remains under investigation, necessitating further research to clarify its physiological and pathological relevance.
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