| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM63A |
| Uniprot No | Q8N5J2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MEYHQPEDPAPGKAGTAEAVIPENHEVLAGPDEHPQDTDARDADGEAREREPADQALLPSQCGDNLESPLPEASSAPPGPTLGTLPEVETIRACSMPQELPQSPRTRQPEPDFYCVKWIPWKGEQTPIITQSTNGPCPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPEKQLQVDQDYLIALSLQQQQPRGPLGLTDLELAQQLQQEEYQQQQAAQPVRMRTRVLSLQGRGATSGRPAGERRQRPKHESDCILL |
| 分子量 | 78.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM63A蛋白的模拟参考文献示例(文献为假设性构造，仅供参考格式)：
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1. **文献名称**: *FAM63A Interacts with Mitochondrial Proteins and Modulates Energy Metabolism in Human Cells*
**作者**: Lee S, et al.
**摘要**: 本研究通过重组表达人FAM63A蛋白，发现其与线粒体内膜蛋白复合物相互作用，调控细胞能量代谢通路。敲低FAM63A导致ATP生成减少，提示其在代谢疾病中的潜在作用。
2. **文献名称**: *Recombinant FAM63A Binds PP1 Phosphatase and Regulates Neuronal Differentiation*
**作者**: Müller T, et al.
**摘要**: 使用重组FAM63A蛋白进行互作实验，证实其通过结合蛋白磷酸酶PP1调控Tau蛋白去磷酸化，影响神经元分化过程，为阿尔茨海默病机制研究提供新方向。
3. **文献名称**: *Structural and Functional Characterization of Human FAM63A in Wnt/β-catenin Signaling*
**作者**: Gupta R, et al.
**摘要**: 解析重组FAM63A蛋白的晶体结构，揭示其通过直接结合β-catenin增强Wnt信号传导，在结直肠癌细胞中促进增殖和迁移。
4. **文献名称**: *FAM63A as a Novel Biomarker in Hepatocellular Carcinoma: Recombinant Protein-Based Analysis*
**作者**: Wang Q, et al.
**摘要**: 重组FAM63A蛋白的高表达与肝癌患者不良预后相关，机制研究表明其通过调控p53泛素化促进肿瘤进展。
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**注意**：上述文献为模拟示例，实际研究中请通过 **PubMed** 或 **Google Scholar** 使用关键词“FAM63A recombinant”或“FAM63A function”查询最新成果。
**Background of Recombinant Human FAM63A Protein**
The FAM63A (Family with sequence similarity 63 member A) protein, also known as C14orf149. is a less-characterized protein encoded by the *FAM63A* gene in humans. It is evolutionarily conserved across species, suggesting potential roles in critical cellular processes. While its precise biological functions remain under investigation, emerging studies link FAM63A to cell cycle regulation, intracellular signaling, and neurodevelopment. For instance, FAM63A has been implicated in interactions with components of the ubiquitin-proteasome system and cell division regulators, hinting at involvement in protein degradation pathways or mitotic control. Dysregulation of FAM63A expression has been observed in certain cancers, neurodegenerative disorders, and developmental conditions, underscoring its potential pathophysiological relevance.
Recombinant human FAM63A protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Purification often involves affinity tags (e.g., His-tag) coupled with chromatography techniques. The recombinant protein facilitates research into its molecular interactions, post-translational modifications, and role in diseases. Structural analyses predict FAM63A to contain conserved phosphorylation sites and disordered regions, which may mediate dynamic protein-protein interactions. Ongoing research aims to delineate its mechanistic contributions to cellular homeostasis and disease, positioning FAM63A as a promising target for therapeutic exploration or biomarker development.
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